Batson Sarah, Rea Dean, Fülöp Vilmos, Roper David I
Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, England.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Apr 1;66(Pt 4):405-8. doi: 10.1107/S1744309110003970. Epub 2010 Mar 31.
A recombinant form of Escherichia coli DdlB (EcDdlB) has been prepared and cocrystallized with ADP and D-alanyl-D-alanine to represent the ternary complex of EcDdlB. Furthermore, EcDdlB has been cocrystallized under the same conditions with the ligands ATP and D-alanyl-D-alanine, representing the product-inhibited complex. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 53.0, b = 97.6, c = 109.5 A and a = 51.2, b = 97.8, c = 110.1 A, respectively, and both contained two molecules in the asymmetric unit. Complete data sets were collected to 1.5 and 1.4 A resolution, respectively, from single crystals under cryogenic conditions using synchrotron radiation.
已制备出重组形式的大肠杆菌DdlB(EcDdlB),并使其与ADP和D-丙氨酰-D-丙氨酸共结晶,以形成EcDdlB的三元复合物。此外,EcDdlB在相同条件下已与配体ATP和D-丙氨酰-D-丙氨酸共结晶,形成产物抑制复合物。晶体属于空间群P2(1)2(1)2(1),其晶胞参数分别为a = 53.0、b = 97.6、c = 109.5 Å和a = 51.2、b = 97.8、c = 110.1 Å,且两个晶胞在不对称单元中均包含两个分子。在低温条件下,使用同步辐射从单晶分别收集了分辨率为1.5 Å和1.4 Å的完整数据集。