Loreille Odile M, Parr Ryan L, McGregor Kevin A, Fitzpatrick Colleen M, Lyon Chriss, Yang Dongya Y, Speller Camilla F, Grimm Michael R, Grimm Michael J, Irwin Jodi A, Robinson Edward M
Armed Forces DNA Identification Laboratory, 1413 Research Blvd., Rockville, MD 20850, USA.
J Forensic Sci. 2010 May;55(3):813-8. doi: 10.1111/j.1556-4029.2010.01356.x. Epub 2010 Apr 2.
This report describes the identification of a merchant mariner who perished in 1948 when Northwest Airlines Flight 4422, a DC-4 carrying 24 seamen and six crew members crashed into Mount Sanford, Alaska. Fifty-one years later, a human forearm and hand were found close by the wreckage of the plane, prompting identification efforts using DNA and fingerprints. There were significant challenges to both the fingerprint and DNA analyses. The hand was badly desiccated, making fingerprint friction-ridge detail almost invisible and the remains had been embalmed upon discovery, making DNA amplification difficult. We present the results of an interdisciplinary approach that successfully addressed these challenges and ultimately led to the identification of the remains. These efforts relied on efficient fingerprint rejuvenation and imaging techniques that improved print resolution, as well as new DNA extraction techniques optimized for aggressively embalmed remains.
本报告描述了一名商船船员的身份鉴定情况。该船员于1948年遇难,当时西北航空公司4422号航班(一架搭载24名海员和6名机组人员的DC - 4型飞机)坠毁在阿拉斯加的圣福德山。51年后,在飞机残骸附近发现了一只人类前臂和手,于是展开了利用DNA和指纹进行身份鉴定的工作。指纹分析和DNA分析都面临着重大挑战。这只手严重脱水,使得指纹摩擦嵴细节几乎不可见,而且遗体在被发现时已进行了防腐处理,这使得DNA扩增变得困难。我们展示了一种跨学科方法的结果,该方法成功应对了这些挑战,并最终确定了遗体身份。这些工作依赖于提高指纹分辨率的高效指纹修复和成像技术,以及针对经过深度防腐处理的遗体优化的新DNA提取技术。