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雌激素处理大鼠子宫组织切片上交感神经突起生长减少。

Reduced sympathetic neurite outgrowth on uterine tissue sections from rats treated with estrogen.

机构信息

Laboratory of Cell Biology, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, Montevideo, 11600, Uruguay.

出版信息

Cell Tissue Res. 2010 May;340(2):287-301. doi: 10.1007/s00441-010-0956-x. Epub 2010 Apr 13.

Abstract

In order to evaluate the contribution of substrate-bound factors to the extent and patterning of the sympathetic innervation of rat uterus following estrogen treatment, superior cervical ganglion explants from neonatal and adult ovariectomized rats were cultured on tissue sections of fresh frozen uterus from adult ovariectomized rats treated with estrogen or a vehicle. The main findings were: (1) neurite growth was greatly influenced by histological features of the underlying section; (2) on myometrial sections, neurites followed the orientation of the main axis of the longitudinally sectioned muscle cells; (3) neurites showed limited growth on transversally sectioned smooth muscle; (4) neuritic patterning was unaffected by a reduction in migrating ganglionic non-neuronal cells; (5) neurite outgrowth, but not non-neural cell migration, was markedly reduced on myometrial sections from rats treated with estrogen. These results suggest that adult myometrium continues to provide signals allowing the organotypic patterning and growth of sympathetic axons, that estrogen treatment modifies myometrial substrate properties so that it is less supportive for sympathetic neurite growth, and that adult sympathetic neurons retain their ability to recognize substrate-bound cues present in the myometrium. On endometrial sections, neurites formed radially symmetric halos, which were reduced in size on estrogen-treated endometrial substrates. Thus, changes in the neuritogenic capacity of the uterus underlie plasticity in uterine sympathetic nerves, and alterations in substrate-bound factors contribute to the diminished receptivity of the estrogenized uterus to its sympathetic innervation.

摘要

为了评估底物结合因子对雌激素处理后大鼠子宫交感神经支配的范围和模式的贡献,我们将新生和成年去卵巢大鼠的颈上交感神经节外植体培养在成年去卵巢大鼠的新鲜冷冻子宫组织切片上,这些大鼠接受了雌激素或载体处理。主要发现如下:(1)神经突的生长受到下伏切片组织学特征的极大影响;(2)在子宫平滑肌切片上,神经突沿着纵向切片的肌肉细胞的主轴方向延伸;(3)神经突在横向切片的平滑肌上生长有限;(4)减少迁移性神经节非神经元细胞不会影响神经突的模式形成;(5)在接受雌激素治疗的大鼠的子宫平滑肌切片上,神经突的生长,但非神经细胞的迁移,明显减少。这些结果表明,成年子宫平滑肌继续提供允许交感神经轴体发生和生长的器官发生模式信号,雌激素处理改变了子宫平滑肌的基质特性,使其对交感神经突生长的支持性降低,成年交感神经元保留其识别存在于子宫平滑肌中的基质结合线索的能力。在内膜切片上,神经突形成了径向对称的晕圈,在接受雌激素处理的子宫内膜基质上,这些晕圈的大小减小。因此,子宫交感神经可塑性的基础是子宫的神经发生能力的变化,而基质结合因子的改变导致雌激素化子宫对其交感神经支配的接受性降低。

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