Jia Shiru, Xu Chunying, Tan Zhilei, Cao Weifeng, Ou Hongyu, He Xinyi, Deng Zixin
School of Biotechnology, Tianjin University of Science & Technology, Key Laboratory of Industrial Microbiology of Ministry of Education, Tianjin 300457, China.
Wei Sheng Wu Xue Bao. 2010 Feb;50(2):191-6.
Epsilon-Poly-L-Lysine (epsilon-PL) is a natural amino acid homopolymer. The study aimed at isolating new epsilon-PL-producing strains.
epsilon-PL-producing strains were screened from the soil by using a new isolation approach which had three steps, (1) enrichment culturing epsilon-PL toleranting strains; (2) screening by improved Nishikawa's method; (3) selection of strains with higher epsilon-PL tolerant ability.
A new epsilon-PL-producing strain TUST-2 was isolated from the soil collected from Hainan province, China. Chemotaxonomic and morphological characteristics of the isolate were typical of strain of the genus Streptomyces. The strain TUST-2 was found to belong to Streptomyces diastatochromogenes by comparative 16S rRNA gene sequence analysis. The purified fermentation product of the strain TUST-2 was confirmed as epsilon-PL by characteristic analysis, hydrolysate analysis, infrared spectrum, 1H NMR Spectrum, 13C NMR Spectrum, and MALDI-TOF-MS.
On the basis of 16S rRNA gene sequence analysis and its morphological and physiological characteristics, epsilon-PL-producing strain TUST-2 is a new isolate of Streptomyces diastatochromogenes, named as Streptomyces diastatochromogenes TUST-2.
ε-聚-L-赖氨酸（ε-PL）是一种天然氨基酸同聚物。本研究旨在分离新的产ε-PL菌株。
采用一种新的分离方法从土壤中筛选产ε-PL菌株，该方法包括三个步骤：（1）富集培养耐ε-PL菌株；（2）用改良的西川法进行筛选；（3）选择耐ε-PL能力更强的菌株。
从中国海南省采集的土壤中分离出一株新的产ε-PL菌株TUST-2。该分离株的化学分类和形态学特征为链霉菌属菌株的典型特征。通过比较16S rRNA基因序列分析，发现菌株TUST-2属于龟裂链霉菌。通过特征分析、水解产物分析、红外光谱、1H NMR光谱、13C NMR光谱和基质辅助激光解吸电离飞行时间质谱，证实菌株TUST-2的纯化发酵产物为ε-PL。
基于16S rRNA基因序列分析及其形态和生理特征，产ε-PL菌株TUST-2是龟裂链霉菌的一个新分离株，命名为龟裂链霉菌TUST-2。
