[BIOMED-2引物在眼附属器淋巴瘤免疫球蛋白基因重排分析中的应用：一项初步研究]
[Application of BIOMED-2 primers in immunoglobulin gene rearrangement analysis of ocular adnexal lymphoma: a pilot study].
作者信息
Wang Yu-chuan, Wang Li-ming, Hao Peng, Ying Ming, Han Rui-fang, Lin Jin-yong
机构信息
Tianjin Eye Institute, Tianjin Eye Hospital, the Ophthalmic College of Tianjin Medical University, China.
出版信息
Zhonghua Bing Li Xue Za Zhi. 2010 Jan;39(1):31-4.
OBJECTIVE
To assess the practical value of BIOMED-2 primers in the diagnosis of ocular adnexal lymphoma by PCR.
METHODS
DNA was extracted from 63 formalin-fixed paraffin-embedded (FFPE) ocular adnexal lymphoma specimens. The DNA quality was evaluated by PCR-based amplification of housekeeping gene beta-actin. IgH(B) and IgK(B) primers of BIOMED-2 standardized clonality analysis system were used to evaluate the immunoglobin gene rearrangements. PCR products were analyzed using capillary electrophoresis and GeneScan software.
RESULTS
76.2% (48/63) of FFPE samples produced amplifiable DNA for detection of Ig gene rearrangements.Positive detection rates by BIOMED-2 IgH(B) and IgK(B) primers were 79.2% (38/48) and 68.8% (33/48), respectively, with a combined positive detection rate of 91.7% (44/48).
CONCLUSIONS
IgH(B) and IgK(B) primers of BIOMED-2 are suitable for the detection of clonal rearrangements of Ig gene using FFPE specimens of ocular adnexal lymphomas.
目的
通过聚合酶链反应（PCR）评估BIOMED-2引物在眼附属器淋巴瘤诊断中的实用价值。
方法
从63例福尔马林固定石蜡包埋（FFPE）的眼附属器淋巴瘤标本中提取DNA。通过基于PCR的管家基因β-肌动蛋白扩增来评估DNA质量。使用BIOMED-2标准化克隆性分析系统的IgH(B)和IgK(B)引物来评估免疫球蛋白基因重排。使用毛细管电泳和GeneScan软件分析PCR产物。
结果
76.2%（48/63）的FFPE样本产生了可用于检测Ig基因重排的可扩增DNA。BIOMED-2 IgH(B)和IgK(B)引物的阳性检测率分别为79.2%（38/48）和68.8%（33/48），联合阳性检测率为91.7%（44/48）。
结论
BIOMED-2的IgH(B)和IgK(B)引物适用于使用眼附属器淋巴瘤的FFPE标本检测Ig基因的克隆重排。
Zotero 插件