Institute for Biotechnology and Bioengineering, Centre of Genetics and Biotechnology, (IBB/CGB-UTAD), Universidade de Trás-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal.
Appl Microbiol Biotechnol. 2010 Jul;87(4):1317-26. doi: 10.1007/s00253-010-2558-7.
Herein, we report the influence of different combinations of initial concentration of acetic acid and ethanol on the removal of acetic acid from acidic wines by two commercial Saccharomyces cerevisiae strains S26 and S29. Both strains reduced the volatile acidity of an acidic wine (1.0 gl(-1) acetic acid and 11% (v/v) ethanol) by 78% and 48%, respectively. Acetic acid removal by strains S26 and S29 was associated with a decrease in ethanol concentration of 0.7 and 1.2% (v/v), respectively. Strain S26 revealed better removal efficiency due to its higher tolerance to stress factors imposed by acidic wines. Sulfur dioxide (SO(2)) in the concentration range 95-170 mg l(-1)inhibits the ability of both strains to reduce the volatile acidity of the acidic wine used under our experimental conditions. Therefore, deacidification should be carried out either in wines stabilized by filtration or in wines with SO(2)concentrations up to 70 mg l(-1). Deacidification of wines with the better performing strain S26 was associated with changes in the concentration of volatile compounds. The most pronounced increase was observed for isoamyl acetate (banana) and ethyl hexanoate (apple, pineapple), with an 18- and 25-fold increment, respectively, to values above the detection threshold. The acetaldehyde concentration of the deacidified wine was 2.3 times higher, and may have a detrimental effect on the wine aroma. Moreover, deacidification led to increased fatty acids concentration, but still within the range of values described for spontaneous fermentations, and with apparently no negative impact on the organoleptical properties.
在此,我们报告了不同初始浓度的乙酸和乙醇组合对两种商业酿酒酵母菌株 S26 和 S29 从酸性葡萄酒中去除乙酸的影响。这两种菌株分别将酸性葡萄酒(1.0 gl(-1)乙酸和 11%(v/v)乙醇)的挥发性酸度降低了 78%和 48%。菌株 S26 和 S29 去除乙酸与乙醇浓度分别降低了 0.7%和 1.2%(v/v)有关。菌株 S26 由于对酸性葡萄酒施加的应激因素具有更高的耐受性,因此显示出更好的去除效率。在我们的实验条件下,浓度范围为 95-170 mg l(-1)的二氧化硫(SO(2))抑制了这两种菌株降低所用酸性葡萄酒挥发性酸度的能力。因此,脱酸应在过滤稳定的葡萄酒或 SO(2)浓度高达 70 mg l(-1)的葡萄酒中进行。使用表现更好的菌株 S26 进行脱酸会导致挥发性化合物浓度发生变化。最显著的增加发生在异戊酸乙酯(香蕉)和己酸乙酯(苹果、菠萝),分别增加了 18 倍和 25 倍,达到检测阈值以上。脱酸葡萄酒中的乙醛浓度高 2.3 倍,可能对葡萄酒香气产生不利影响。此外,脱酸导致脂肪酸浓度增加,但仍在自发发酵描述的范围内,并且显然对感官特性没有负面影响。