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一种分析墨西哥钝口螈中有机磷农药残留的方法。

A method for the analysis of organophosphorus pesticide residues in Mexican axolotl.

机构信息

Facultad de Química de la Universidad Nacional Autónoma de Mexico (UNAM), México, D.F., México.

出版信息

J Environ Sci Health B. 2010 Jan;45(1):25-32. doi: 10.1080/03601230903404309.

Abstract

A method based on matrix solid-phase dispersion (MSPD) was developed for quantitative extraction of three organophosphorus pesticides (OPPs) from the Mexican axolotl, Ambystoma mexicanum. The determination was carried out using high- performance liquid chromatography (HPLC) with diode array spectrophotometric UV detection (DAD). The MSPD extraction with octadecylsilyl (C18) sorbent combined with a silica gel clean-up and acetonitrile elution was optimised for chlorpyrifos, fenthion and methyl parathion. The method was validated, yielding recovery values higher than 90%. The precision, expressed as the relative standard deviation (RSD), was less than or equal to 6% in muscle samples at spiking levels of 10 and 5 ppm. Linearity was studied from 15 to 60 ppm for chlorpyrifos and fenthion, and from 7.5 to 30 ppm for methyl parathion. The limits of detection (LODs) were found to be less than or equal to 0.5 ppm. This method was applied to the analysis of samples from a chlorpyrifos-exposed axolotl, demonstrating its use as an analytical tool for toxicological studies.

摘要

基于基质固相分散(MSPD)的方法被开发用于从墨西哥钝口螈(Ambystoma mexicanum)中定量提取三种有机磷农药(OPPs)。使用高效液相色谱(HPLC)与二极管阵列分光光度紫外检测(DAD)进行测定。用十八烷基硅(C18)吸附剂进行 MSPD 提取,结合硅胶净化和乙腈洗脱,对氯吡硫磷、倍硫磷和甲基对硫磷进行了优化。该方法经过验证,回收率高于 90%。在肌肉样品中,10 和 5 ppm 加标水平下的精密度(表示为相对标准偏差(RSD))小于或等于 6%。线性范围为氯吡硫磷和倍硫磷的 15 至 60 ppm,甲基对硫磷的 7.5 至 30 ppm。检出限(LOD)小于或等于 0.5 ppm。该方法应用于暴露于氯吡硫磷的钝口螈样品的分析,证明其可作为毒理学研究的分析工具。

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