Chand Karam, Biswas Sanchay K, Sing Baneswar, De Ankan, Mondal Bimalendu
Division of Virology, Indian Veterinary Research Institute, Mukteswar Campus, Dist. Nainital 263 138, Uttarakhand, India.
Vet Ital. 2009 Jul-Sep;45(3):443-8.
A polyclonal antibody-based sandwich enzyme-linked immunosorbent assay (s-ELISA) was developed for the detection of bluetongue virus (BTV). The test used antiserum against BTV and antiserum against the bluetongue (BT) core protein. The antiserum against the virus was used as a capture antibody and the antiserum against the protein was used for detection. In this study, antiserum to recombinant viral protein 7 (rVP7) was used as a detection antibody in place of anti-core antiserum. The assay was used to detect the BT serotypes found in India, namely: 1, 2, 9, 15, 18 and 23. The modified sandwich assay was able to detect BTV serotypes in cell culture supernatants. The use of anti-rVP7 antiserum as the detection antibody avoids the tedious and expensive purification of BTV core particles.
开发了一种基于多克隆抗体的夹心酶联免疫吸附测定法（s-ELISA）用于检测蓝舌病毒（BTV）。该检测使用了抗BTV抗血清和抗蓝舌病（BT）核心蛋白抗血清。抗病毒抗血清用作捕获抗体，抗蛋白抗血清用于检测。在本研究中，使用针对重组病毒蛋白7（rVP7）的抗血清代替抗核心抗血清作为检测抗体。该测定法用于检测在印度发现的BT血清型，即：1、2、9、15、18和23。改良的夹心测定法能够检测细胞培养上清液中的BTV血清型。使用抗rVP7抗血清作为检测抗体避免了繁琐且昂贵的BTV核心颗粒纯化过程。
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