在亚得里亚海（意大利）的可食牡蛎中检测卵形贝内孢菌和出口贝内孢菌（单孢子虫纲）
Detection of Bonamia ostreae and B. exitiosa (Haplosporidia) in Ostrea edulis from the Adriatic Sea (Italy).
作者信息
Narcisi V, Arzul I, Cargini D, Mosca F, Calzetta A, Traversa D, Robert M, Joly J P, Chollet B, Renault T, Tiscar P G
机构信息
Department of Comparative Biomedical Sciences, University of Teramo, P. A. Moro 45, 64100, Teramo, Italy.
出版信息
Dis Aquat Organ. 2010 Feb 24;89(1):79-85. doi: 10.3354/dao02167.
The flat oyster Ostrea edulis L. is widespread along the Italian coasts. In particular, the Manfredonia Gulf (Adriatic Sea) represents an important site where natural beds subsist. Previous monitoring conducted in 1990 by light microscopy and ultrastructural studies revealed the presence of Bonamia-like microcell parasites in some flat oysters: following this observation, a new sampling of O. edulis was carried out at this location in 2007. Of 750 oysters collected, 3 showed the presence of uninucleated microcells (2 to 3 microm diameter) free or inside the haemocyte cytoplasm by cytology and histopathology. Molecular analysis confirmed that the microcells in 2 oysters were B. exitiosa, whereas in the third oyster the microcells were B. ostreae. Moreover, molecular studies were carried out to confirm the existence of Bonamia sp. in archived samples, confirming the presence of B. ostreae in the Manfredonia Gulf since 1990.
欧洲平牡蛎（Ostrea edulis L.）在意大利沿海广泛分布。特别是曼弗雷多尼亚湾（亚得里亚海）是自然牡蛎床仍然存在的一个重要地点。1990年通过光学显微镜和超微结构研究进行的先前监测显示，一些欧洲平牡蛎中存在类博纳介虫微细胞寄生虫：基于这一观察结果，2007年在该地点对欧洲平牡蛎进行了新的采样。在采集的750只牡蛎中，有3只通过细胞学和组织病理学检查显示存在单核微细胞（直径2至3微米），这些微细胞游离于血细胞细胞质中或存在于血细胞细胞质内。分子分析证实，2只牡蛎中的微细胞为出芽博纳介虫（B. exitiosa），而第三只牡蛎中的微细胞为牡蛎博纳介虫（B. ostreae）。此外，还进行了分子研究以确认存档样本中博纳介虫属（Bonamia sp.）的存在，证实自1990年以来曼弗雷多尼亚湾就存在牡蛎博纳介虫。
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