State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province, China.
Nutrition. 2010 Oct;26(10):1008-13. doi: 10.1016/j.nut.2009.11.011. Epub 2010 Apr 14.
To clarify the adhesion mechanism of Lactobacillus acidophilus to Peyer's patches.
Adhesion of L. acidophilus FN001 to mice Peyer's patches was studied in vitro using a fluorescent quantization method. The nature of adhesion mediator was studied by the differing effects of physical, chemical, and enzymatic pre-treatments of the bacteria and the inhibitory effects of sugars on the adhesion. The presence of lectin-like proteins on the cell surface was determined by hemagglutination assay. The effect of L. acidophilus FN001 on the inhibition of adhesion of pathogens to Peyer's patches was also studied.
The adhesion of L. acidophilus FN001 was strongly inhibited in the presence of D-mannose and methyl-α-D-mannoside. Pretreatment of L. acidophilus FN001 with pepsin and trypsin decreased the adhesive capacity indicating that some cell surface proteins might be involved in the adhesion. L. acidophilus FN001 showed agglutinating activity toward the rabbit red cells in a mannose specific manner, which was decreased after protease pretreatment, suggesting possible occurrence of mannose specific lectin(s) on the L. acidophilus FN001 surface. In adhesion inhibition assay, L. acidophilus NF001, when applied to Peyer's patches first or at the same time with pathogen, significantly inhibited adhesion of Escherichia coli ATCC25922 to Peyer's patches.
L. acidophilus FN001 contains some mannose-specific protein(s) on its surface that mediates its adhesion to the Peyer's patches. FN001 inhibits the adhesion of E. coli, which also contains mannose specific lectin.
阐明嗜酸乳杆菌对派尔集合淋巴结的黏附机制。
采用荧光定量法研究嗜酸乳杆菌 FN001 体外黏附小鼠派尔集合淋巴结的情况。通过细菌的物理、化学和酶预处理的不同影响以及糖对黏附的抑制作用研究黏附介质的性质。通过血凝试验确定细胞表面是否存在凝集素样蛋白。还研究了嗜酸乳杆菌 FN001 对抑制病原体黏附派尔集合淋巴结的影响。
在 D-甘露糖和甲基-α-D-甘露糖苷存在的情况下，嗜酸乳杆菌 FN001 的黏附受到强烈抑制。用胃蛋白酶和胰蛋白酶预处理嗜酸乳杆菌 FN001 会降低其黏附能力，表明某些细胞表面蛋白可能参与了黏附。嗜酸乳杆菌 FN001 以甘露糖特异性的方式对兔红细胞表现出凝集活性，经蛋白酶预处理后活性降低，表明嗜酸乳杆菌 FN001 表面可能存在甘露糖特异性凝集素。在黏附抑制试验中，嗜酸乳杆菌 NF001 首先或与病原体同时应用于派尔集合淋巴结时，可显著抑制大肠杆菌 ATCC25922 与派尔集合淋巴结的黏附。
嗜酸乳杆菌 FN001 表面含有一些甘露糖特异性蛋白，介导其与派尔集合淋巴结的黏附。FN001 抑制含有甘露糖特异性凝集素的大肠杆菌的黏附。
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