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通过RNA干扰在治愈持续性病毒感染的细胞中增强基因沉默。

Enhanced gene silencing in cells cured of persistent virus infection by RNA interference.

作者信息

Pelletier Isabelle, Saulnier Aure, Brisac Cynthia, Jegouic Sophie, Vabret Nicolas, Tangy Frédéric, Blondel Bruno, Colbère-Garapin Florence

机构信息

Institut Pasteur, Unité de Biologie des virus entériques, 25 rue du Dr Roux, 75015 Paris, France.

出版信息

J Virol. 2010 Jul;84(13):6880-5. doi: 10.1128/JVI.02060-09. Epub 2010 Apr 14.

DOI:10.1128/JVI.02060-09
PMID:20392843
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2903240/
Abstract

We compared HEp-2-derived cells cured of persistent poliovirus infection by RNA interference (RNAi) with parental cells, to investigate possible changes in the efficiency of RNAi. Lower levels of poliovirus replication were observed in cured cells, possibly facilitating virus silencing by antiviral small interfering RNAs (siRNAs). However, green fluorescent protein (GFP) produced from a measles virus vector and also GFP and luciferase produced from plasmids that do not replicate in human cells were more effectively silenced by specific siRNAs in cured than in control cells. Thus, cells displaying enhanced silencing were selected during curing by RNAi. Our results strongly suggest that the RNAi machinery of cured cells is more efficient than that of parental cells.

摘要

我们将通过RNA干扰（RNAi）治愈持续性脊髓灰质炎病毒感染的HEp-2衍生细胞与亲代细胞进行比较，以研究RNAi效率可能发生的变化。在治愈的细胞中观察到较低水平的脊髓灰质炎病毒复制，这可能有助于抗病毒小干扰RNA（siRNA）使病毒沉默。然而，由麻疹病毒载体产生的绿色荧光蛋白（GFP），以及由不在人类细胞中复制的质粒产生的GFP和荧光素酶，在治愈的细胞中比在对照细胞中更能被特异性siRNA有效沉默。因此，在通过RNAi治愈的过程中选择了显示出增强沉默作用的细胞。我们的结果有力地表明，治愈细胞的RNAi机制比亲代细胞的更有效。

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