School of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea.
EMBO Rep. 2010 May;11(5):380-6. doi: 10.1038/embor.2010.44. Epub 2010 Apr 16.
Imperfect base-pairing between microRNA (miRNA) and the 3'-untranslated region of target messenger RNA (mRNA) triggers translational repression of the target mRNA. Here, we provide evidence that human Argonaute 2 targets cap-binding protein (CBP)80/20-bound mRNAs and exon junction complex-bound mRNAs and inhibits nonsense-mediated mRNA decay (NMD), which is restricted tightly to CBP80/20-bound mRNAs. Furthermore, microarray analyses reveal that a subset of cellular transcripts, which are expected to be targeted for NMD, is stabilized by miRNA-mediated gene silencing. The regulation of NMD by miRNAs will shed light on a new post-transcriptional regulation mechanism of gene expression in mammalian cells.
miRNA 与靶信使 RNA(mRNA)的 3'-非翻译区之间的不完全碱基配对会触发靶 mRNA 的翻译抑制。在这里,我们提供的证据表明,人 Argonaute 2 靶向帽结合蛋白(CBP)80/20 结合的 mRNA 和外显子连接复合物结合的 mRNA,并抑制无意义介导的 mRNA 降解(NMD),NMD 严格局限于 CBP80/20 结合的 mRNA。此外,微阵列分析显示,一组预期被 NMD 靶向的细胞转录本,通过 miRNA 介导的基因沉默而稳定。miRNA 对 NMD 的调节将阐明哺乳动物细胞中基因表达的新的转录后调控机制。