Department of Animal Science, North Carolina State University, Raleigh, Raleigh, NC 27695-7621, USA.
Domest Anim Endocrinol. 2010 Aug;39(2):90-6. doi: 10.1016/j.domaniend.2010.02.006. Epub 2010 Mar 20.
Ghrelin is a peptide hormone that has been implicated in the regulation of feed intake, but little is known about its secretion in pigs. Hence, the effect of feeding pattern on the regulation of ghrelin secretion was tested. In experiment 1, barrows were allotted randomly into 1 of 2 groups, (1) ad libitum fed (CONT) and (2) limited access to feed (once per day, MEAL). Blood samples were taken through jugular catheters every 15 min for 6 h after 7 d on the experimental feeding regimen. Plasma concentrations of ghrelin and insulin were determined by radioimmunoassay. Ghrelin concentrations in the MEAL pigs were elevated before feeding and declined after feeding (P < 0.01). No pattern in plasma ghrelin concentrations was observed in the CONT pigs, but ghrelin concentrations were lower than in the MEAL group. Insulin concentrations were greater in CONT pigs (P < 0.01) during most of the sampling and increased after feeding in the MEAL pigs (P < 0.01). In experiment 2, the treatments were the same as in experiment 1; however, the amount of feed was increased in the MEAL group so that their daily intake was similar to the CONT pigs. Ghrelin concentrations in the MEAL group were again elevated before the meal and declined afterward (P < 0.01). Insulin but not glucose concentrations were negatively correlated with ghrelin. Once-per-day feeding resulted in increased plasma concentrations of ghrelin, which decreased after feeding. Ghrelin may be involved in the regulation of feed intake in pigs.
胃饥饿素是一种肽类激素，它与采食量的调节有关，但关于其在猪中的分泌知之甚少。因此，本试验研究了采食方式对胃饥饿素分泌的调节作用。试验 1 将公猪随机分为 2 组，（1）自由采食（CONT）和（2）限时采食（每天 1 次，MEAL）。在试验饲粮条件下适应 7 d 后，通过颈静脉导管每隔 15 min 采集 1 次血样，持续 6 h。采用放射免疫法测定血浆胃饥饿素和胰岛素浓度。MEAL 组猪的胃饥饿素浓度在采食前升高，采食后降低（P < 0.01）。CONT 组猪的血浆胃饥饿素浓度没有明显变化规律，但浓度低于 MEAL 组。CONT 组猪的胰岛素浓度在大部分采样时间点均高于 MEAL 组（P < 0.01），MEAL 组猪的胰岛素浓度在采食后增加（P < 0.01）。试验 2 的处理与试验 1 相同，但增加了 MEAL 组的饲料量，以使它们的日采食量与 CONT 组相似。MEAL 组的胃饥饿素浓度再次在采食前升高，采食后降低（P < 0.01）。胰岛素而不是葡萄糖浓度与胃饥饿素呈负相关。每日 1 次的采食导致血浆胃饥饿素浓度升高，采食后降低。胃饥饿素可能参与了猪的采食量调节。
