Department of Pediatrics, Yamaguchi University Graduate School of Medicine, Japan.
Cell Immunol. 2010;263(2):161-5. doi: 10.1016/j.cellimm.2010.03.010. Epub 2010 Mar 29.
Beta1-integrins mediate cell attachment to different extracellular matrix proteins, intracellular proteins, and intercellular adhesions. Recently, it has been reported that prostaglandin E2 (PGE2) has anti-inflammatory properties such as inhibition of the expression of adhesion molecules or production of chemokines. However, the effect of PGE2 on the expression of beta1-integrin remains unknown. In this study, we investigated the effects of PGE2 on the expression of beta1-integrin in the human monocytic cell line THP-1 and in CD14+ monocytes/macrophages in human peripheral blood. For this, we examined the role of four subtypes of PGE2 receptors and E-prostanoid (EP) receptors on PGE2-mediated inhibition. We found that PGE2 significantly inhibited the expression of beta1-integrin, mainly through EP4 receptors in THP-1 cells and CD14+ monocytes/macrophages in human peripheral blood. We suggest that PGE2 has anti-inflammatory effects, leading to the inhibited expression of beta1-integrin in human monocytes/macrophages, and that the EP4 receptor may play an important role in PGE2-mediated inhibition.
β1 整合素介导细胞附着到不同的细胞外基质蛋白、细胞内蛋白和细胞间黏附。最近有报道称，前列腺素 E2（PGE2）具有抗炎特性，如抑制黏附分子的表达或趋化因子的产生。然而，PGE2 对β1 整合素表达的影响尚不清楚。在这项研究中，我们研究了 PGE2 对人单核细胞系 THP-1 和人外周血中 CD14+单核细胞/巨噬细胞中β1 整合素表达的影响。为此，我们研究了 PGE2 介导的抑制作用中四种亚型的 PGE2 受体和 E-前列腺素（EP）受体的作用。我们发现 PGE2 显著抑制β1 整合素的表达，主要通过 THP-1 细胞和人外周血中 CD14+单核细胞/巨噬细胞中的 EP4 受体。我们认为 PGE2 具有抗炎作用，导致人单核细胞/巨噬细胞中β1 整合素的表达受到抑制，而 EP4 受体可能在 PGE2 介导的抑制中发挥重要作用。
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