The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen, Fujian, 361021, China; State Key Laboratory of Marine Environmental Science, Xiamen University, Environmental Science Research Center, Xiamen University, Xiamen, Fujian, 361005, China.
The Key Laboratory of Science and Technology for Aquaculture and Food Safety, Fisheries College, Jimei University, Xiamen, Fujian, 361021, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2009 Dec;4(4):255-262. doi: 10.1016/j.cbd.2009.06.002. Epub 2009 Jun 21.
The analysis of expressed sequence tags (ESTs) is an efficient approach for gene discovery, expression profiling, and the development of resources for functional genomics. To analyze the transcriptome response to tributyltin of small abalone Haliotis diversicolor supertexta, a normalized cDNA library of hepatopancreas after exposure of animals to tributyltin was constructed. Three thousand and forty eight high quality ESTs were generated. After processing, a total of 2473 unigenes comprising 370 contigs and 2103 singlets were acquired. BLAST identified 1108 clones (45%) as known genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Simple Sequence Repeat (SSR) identification were also carried out to acquire more information on pathway and EST-SSR. According to GO categories, the total ESTs distribute across the various functional categories. In addition, 43 potential toxicology-related clusters were identified. This work provides the first set of genetic data for small abalone which is of great value for further exploitation of this species in functional genomics and toxicogenomics. Our interesting results will be mainly useful to choose biomarkers for ecotoxicological studies.
表达序列标签（ESTs）分析是一种有效的基因发现、表达谱分析和功能基因组学资源开发方法。为了分析三丁基锡对小型鲍螺 Haliotis diversicolor supertexta 转录组的反应，我们构建了动物暴露于三丁基锡后肝胰腺的归一化 cDNA 文库。生成了 3048 个高质量的 EST。经过处理，共获得 2473 个基因，包括 370 个重叠群和 2103 个单基因。BLAST 将 1108 个克隆（45%）鉴定为已知基因。京都基因与基因组百科全书（KEGG）和简单重复序列（SSR）鉴定也进行了，以获得更多关于途径和 EST-SSR 的信息。根据 GO 类别，总 EST 分布在各种功能类别中。此外，还鉴定了 43 个潜在的毒理学相关簇。这项工作为小型鲍螺提供了第一组遗传数据，这对于进一步在功能基因组学和毒理学基因组学中开发该物种具有重要价值。我们的有趣结果将主要有助于选择生态毒理学研究的生物标志物。
Zotero 插件