消除神经肌肉自身免疫性血清检测中的假阳性结果。

Eliminating false-positive results in serum tests for neuromuscular autoimmunity.

机构信息

Department of Laboratory Medicine and Pathology, Mayo Clinic College of Medicine, Rochester, Minnesota, USA.

出版信息

Muscle Nerve. 2010 May;41(5):702-4. doi: 10.1002/mus.21653.

PMID:20405502

Abstract

In this study we describe the false-positive frequency in radioimmunoprecipitation assays for muscle acetylcholine receptor (AChR) and neuronal voltage-gated potassium channel (VGKC) autoantibodies, attributable to 125I-ligand immunoprecipitation. Sera were evaluated for AChR autoantibody (n = 34,095) and VGKC autoantibody (n = 11,028). We retested sera that yielded apparently positive results with 125I-ligand with and without detergent-solubilized cation-channel protein, indentified clinically validated fals-positive rates of 0.05% and 1.7% for AchR and VGKC autoantibodies, respectively. Specificity assurance in radioimmunoprecipitation assays requires subtraction of values for 125I-ligand binding.

摘要

在这项研究中，我们描述了由于 125I-配体免疫沉淀导致的肌乙酰胆碱受体（AChR）和神经元电压门控钾通道（VGKC）自身抗体的放射免疫沉淀检测中的假阳性频率。评估了 34095 份 AChR 自身抗体血清和 11028 份 VGKC 自身抗体血清。我们用去污剂溶解的阳离子通道蛋白重新检测了那些与 125I-配体明显呈阳性的血清，结果发现 AchR 和 VGKC 自身抗体的假阳性率分别为 0.05%和 1.7%。放射免疫沉淀检测中的特异性保证需要减去 125I-配体结合值。