Departament de Microbiologia, Universitat de Barcelona, Barcelona, Spain.
J Appl Microbiol. 2010 Sep;109(3):1027-34. doi: 10.1111/j.1365-2672.2010.04725.x.
To determine the prevalence of Campylobacter in surface waters of a highly populated Mediterranean area.
Surface water and wastewater samples were collected from an area in the north-east of Spain during a 2-year study. All the samples were analysed using the MPN method and Multiplex PCR to quantify and identify Campylobacter. It was detected in 82% of the samples from the Llobregat River with a mean of 1·3 MPN 100 ml(-1). The lowest counts were obtained in summer. Campylobacter coli was the predominant species in this river. The bacteria were isolated from marsh water but not from seawater samples. The highest counts of campylobacters were found in poultry wastewater where Camp. jejuni was the predominant species, as in urban sewage. In pig slurry, Camp. coli was the only species detected.
Campylobacter jejuni and Camp. coli are present and widely distributed in the surface water of the studied area. The two species co-exist, with Camp. coli being predominant. In river water, campylobacter counts presented a seasonal distribution. No relationship with faecal indicators was found.
This study provides the first data on the occurrence and concentrations of thermotolerant campylobacter species in surface water in a Mediterranean area.
确定人口稠密的地中海地区地表水中弯曲杆菌的流行情况。
在为期两年的研究中，从西班牙东北部的一个地区采集地表水样和污水样。所有样本均采用 MPN 法和多重 PCR 进行分析，以定量和鉴定弯曲杆菌。在 82%的 Llobregat 河样本中检测到弯曲杆菌，平均 100ml(-1)有 1.3 MPN。夏季检出率最低。该河以大肠弯曲杆菌为主。细菌分离自沼泽水，而不是海水样本。在禽畜废水中发现了最高数量的弯曲杆菌，其中以空肠弯曲杆菌为主，与城市污水相同。在猪粪浆中，仅检测到大肠弯曲杆菌。
空肠弯曲杆菌和大肠弯曲杆菌存在并广泛分布于研究地区的地表水中。两种菌共同存在，以大肠弯曲杆菌为主。在河水中，弯曲杆菌的数量呈现季节性分布。未发现与粪便指标有关。
本研究首次提供了地中海地区地表水中耐热弯曲杆菌属物种的发生和浓度数据。
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