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大鼠部分肝切除术后腹腔内空间对肝脏再生的影响。

The impact of the intra-abdominal space on liver regeneration after a partial hepatectomy in rats.

机构信息

Department of Surgery, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.

出版信息

J Surg Res. 2011 Nov;171(1):259-65. doi: 10.1016/j.jss.2010.01.012. Epub 2010 Feb 4.

Abstract

BACKGROUND

Little is known about the relationship between intra-abdominal space and liver regeneration. The present study was experimentally designed to investigate the influence of the "occupied space" or the "loss of occupied space" on a regenerating liver.

METHODS

Experiment 1: Rats were randomly assigned to two groups: SO (space occupied) rats (n = 40); occupancy of intra-abdominal space followed by a two-thirds partial hepatectomy (PH) and control rats (n = 40); A PH alone. The rats in both groups were euthanized at 24, 48, 96, and 168 h after the operation. Computed tomography (CT) images were analyzed to evaluate the regenerating-direction and the shape of the regenerated remnant liver. The liver to body weight ratio and the proliferating cell nuclear antigen (PCNA) labeling index were measured at each time point. Experiment 2: A second laparotomy was performed at 168 h after the PH in both groups; occupier-removal for the SO rats and a sham operation for the control rats. The rats in both groups were euthanized at 24 and 168 h after the second operation. The liver to body weight ratio and PCNA labeling index were measured at each time point.

RESULTS

Experiment 1: The remnant liver of the SO rats enlarged toward the dorsal and caudal side because liver regeneration toward the ventral side in the SO rats was inhibited with the occupier in the abdominal space at 96 h, and later, after the PH. CT images showed a statistically significant difference in the shape of the regenerated remnant liver between the control group and the SO group. The liver/body weight ratio was significantly decreased in the SO rats at 96 and 168 h after PH (P < 0.05). There was no significant difference between the groups in the PCNA labeling index. The SO rats showed a significant increase of the PCNA labeling of the inferior right lobe (10.6%) in comparison with the index of the superior right lobe (7.8%), which came in direct contact with the occupier, at 96 h after the operation (P < 0.05). The cell density of superior right lobe of the SO rats group was significantly higher than that of the control group at 168 h after operation (P < 0.05). Experiment 2: There was no statistically significant difference in the liver/body weight ratio at 168 hrs after the second operation between the groups. However, there was a statistically significant increase of the PCNA labeling index 24 h after the second operation in the occupier-removal rats in comparison with the control rats (P < 0.05).

CONCLUSION

The occupied intra-abdominal space was therefore found to suppress liver regeneration after a partial hepatectomy, while the removal of such an occupied space stimulated the regeneration of the liver.

摘要

背景

关于腹腔内空间与肝再生之间的关系知之甚少。本研究通过实验设计,旨在探讨“占位空间”或“占位空间丢失”对再生肝脏的影响。

方法

实验 1:大鼠随机分为两组:SO(空间占位)组(n=40);腹腔空间占位后行 2/3 肝部分切除术(PH),对照组(n=40);仅行 PH。两组大鼠分别于术后 24、48、96 和 168 小时处死。通过计算机断层扫描(CT)图像分析评估再生方向和再生残余肝脏的形状。在每个时间点测量肝/体重比和增殖细胞核抗原(PCNA)标记指数。实验 2:两组大鼠在 PH 后 168 小时行第二次剖腹术;SO 大鼠占位去除,对照组大鼠假手术。两组大鼠分别于第二次手术后 24 和 168 小时处死。在每个时间点测量肝/体重比和 PCNA 标记指数。

结果

实验 1:SO 大鼠的残余肝脏向背部和尾部方向增大,因为 SO 大鼠的肝再生向腹侧受到抑制,而在 PH 后腹腔内的占位物仍存在。CT 图像显示,对照组和 SO 组之间再生残余肝脏的形状存在统计学显著差异。PH 后 96 和 168 小时,SO 大鼠的肝/体重比明显下降(P<0.05)。两组间 PCNA 标记指数无显著差异。与上右叶(7.8%)相比,SO 大鼠在术后 96 小时,与占位物直接接触的右下叶 PCNA 标记指数显著增加(10.6%)(P<0.05)。SO 大鼠右肝上叶的细胞密度在术后 168 小时明显高于对照组(P<0.05)。实验 2:第二次手术后 168 小时,两组间肝/体重比无统计学显著差异。然而,第二次手术后 24 小时,占位物去除组的 PCNA 标记指数明显高于对照组(P<0.05)。

结论

因此,发现腹腔内占位空间会抑制部分肝切除术后的肝再生,而去除这种占位空间会刺激肝脏再生。

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