成肌细胞 microRNA 表达需要 ATP 依赖的染色质重塑酶功能。
Myogenic microRNA expression requires ATP-dependent chromatin remodeling enzyme function.
机构信息
Department of Cell Biology, University of Massachusetts Medical School, Worcester, MA 01655, USA.
出版信息
Mol Cell Biol. 2010 Jul;30(13):3176-86. doi: 10.1128/MCB.00214-10. Epub 2010 Apr 26.
Abstract
Knockdown of the Brg1 ATPase subunit of SWI/SNF chromatin remodeling enzymes in developing zebrafish caused stunted tail formation and altered sarcomeric actin organization, which phenocopies the loss of the microRNA processing enzyme Dicer, or the knockdown of myogenic microRNAs. Furthermore, myogenic microRNA expression and differentiation was blocked in Brg1 conditional myoblasts differentiated ex vivo. The binding of Brg1 upstream of myogenic microRNA sequences correlated with MyoD binding and accessible chromatin structure in satellite cells and myofibers, and it was required for chromatin accessibility and microRNA expression in a tissue culture model for myogenesis. The results implicate ATP-dependent chromatin remodelers in myogenic microRNA gene regulation.
摘要
在发育中的斑马鱼中敲低 SWI/SNF 染色质重塑酶的 Brg1 ATP 酶亚基导致尾巴形成受阻，并改变了肌节肌动蛋白的组织，这类似于微 RNA 加工酶 Dicer 的缺失，或肌源性微 RNA 的敲低。此外，在体外分化的 Brg1 条件性成肌细胞中，肌源性微 RNA 的表达和分化被阻断。Brg1 在肌源性微 RNA 序列的上游结合与卫星细胞和成肌纤维中的 MyoD 结合和可及染色质结构相关，并且在成肌细胞的组织培养模型中，它对于染色质可及性和微 RNA 表达是必需的。结果表明，ATP 依赖性染色质重塑因子参与肌源性微 RNA 基因调控。
相似文献
1
Myogenic microRNA expression requires ATP-dependent chromatin remodeling enzyme function.
Mol Cell Biol. 2010 Jul;30(13):3176-86. doi: 10.1128/MCB.00214-10. Epub 2010 Apr 26.
2
The SWI/SNF chromatin remodeling complex regulates myocardin-induced smooth muscle-specific gene expression.
Arterioscler Thromb Vasc Biol. 2009 Jun;29(6):921-8. doi: 10.1161/ATVBAHA.109.187229. Epub 2009 Apr 2.
3
Brg1 Controls the Expression of Pax7 to Promote Viability and Proliferation of Mouse Primary Myoblasts.
J Cell Physiol. 2015 Dec;230(12):2990-7. doi: 10.1002/jcp.25031.
4
Calcineurin Broadly Regulates the Initiation of Skeletal Muscle-Specific Gene Expression by Binding Target Promoters and Facilitating the Interaction of the SWI/SNF Chromatin Remodeling Enzyme.
Mol Cell Biol. 2019 Sep 11;39(19). doi: 10.1128/MCB.00063-19. Print 2019 Oct 1.
5
The Bromodomains of the mammalian SWI/SNF (mSWI/SNF) ATPases Brahma (BRM) and Brahma Related Gene 1 (BRG1) promote chromatin interaction and are critical for skeletal muscle differentiation.
Nucleic Acids Res. 2021 Aug 20;49(14):8060-8077. doi: 10.1093/nar/gkab617.
6
The expression of myogenic microRNAs indirectly requires protein arginine methyltransferase (Prmt)5 but directly requires Prmt4.
Nucleic Acids Res. 2011 Mar;39(4):1243-55. doi: 10.1093/nar/gkq896. Epub 2010 Oct 14.
7
Skeletal muscle specification by myogenin and Mef2D via the SWI/SNF ATPase Brg1.
EMBO J. 2006 Feb 8;25(3):490-501. doi: 10.1038/sj.emboj.7600943. Epub 2006 Jan 19.
8
Distinct protein arginine methyltransferases promote ATP-dependent chromatin remodeling function at different stages of skeletal muscle differentiation.
Mol Cell Biol. 2009 Apr;29(7):1909-21. doi: 10.1128/MCB.00742-08. Epub 2009 Feb 2.
9
Casein kinase 2-mediated phosphorylation of Brahma-related gene 1 controls myoblast proliferation and contributes to SWI/SNF complex composition.
J Biol Chem. 2017 Nov 10;292(45):18592-18607. doi: 10.1074/jbc.M117.799676. Epub 2017 Sep 22.
10
Myogenin and the SWI/SNF ATPase Brg1 maintain myogenic gene expression at different stages of skeletal myogenesis.
J Biol Chem. 2007 Mar 2;282(9):6564-70. doi: 10.1074/jbc.M608898200. Epub 2006 Dec 27.
引用本文的文献
1
Differential Contributions of mSWI/SNF Chromatin Remodeler Sub-Families to Myoblast Differentiation.
Int J Mol Sci. 2023 Jul 9;24(14):11256. doi: 10.3390/ijms241411256.
2
Differential requirements for different subfamilies of the mammalian SWI/SNF chromatin remodeling enzymes in myoblast cell cycle progression and expression of the Pax7 regulator.
Biochim Biophys Acta Gene Regul Mech. 2022 Feb;1865(2):194801. doi: 10.1016/j.bbagrm.2022.194801. Epub 2022 Feb 23.
3
The SWI/SNF complex regulates the expression of miR-222, a tumor suppressor microRNA in lung adenocarcinoma.
Hum Mol Genet. 2021 Nov 16;30(23):2263-2271. doi: 10.1093/hmg/ddab187.
4
Non-Coding RNAs and Nucleosome Remodeling Complexes: An Intricate Regulatory Relationship.
Biology (Basel). 2020 Aug 7;9(8):213. doi: 10.3390/biology9080213.
5
CK2-Dependent Phosphorylation of the Brg1 Chromatin Remodeling Enzyme Occurs during Mitosis.
Int J Mol Sci. 2020 Jan 30;21(3):923. doi: 10.3390/ijms21030923.
6
SMARCD2 subunit of SWI/SNF chromatin-remodeling complexes mediates granulopoiesis through a CEBPɛ dependent mechanism.
Nat Genet. 2017 May;49(5):753-764. doi: 10.1038/ng.3812. Epub 2017 Apr 3.
7
ATP-dependent chromatin remodeling during mammalian development.
Development. 2016 Aug 15;143(16):2882-97. doi: 10.1242/dev.128892.
8
SWI/SNF-directed stem cell lineage specification: dynamic composition regulates specific stages of skeletal myogenesis.
Cell Mol Life Sci. 2016 Oct;73(20):3887-96. doi: 10.1007/s00018-016-2273-3. Epub 2016 May 20.
9
Brahma is required for cell cycle arrest and late muscle gene expression during skeletal myogenesis.
EMBO Rep. 2015 Aug;16(8):1037-50. doi: 10.15252/embr.201540159. Epub 2015 Jul 1.
10
Telomerase reverse transcriptase regulates microRNAs.
Int J Mol Sci. 2015 Jan 6;16(1):1192-208. doi: 10.3390/ijms16011192.
本文引用的文献
1
Chromatin: the interface between extrinsic cues and the epigenetic regulation of muscle regeneration.
Trends Cell Biol. 2009 Jun;19(6):286-94. doi: 10.1016/j.tcb.2009.03.002. Epub 2009 Apr 23.
2
Zebrafish miR-1 and miR-133 shape muscle gene expression and regulate sarcomeric actin organization.
Genes Dev. 2009 Mar 1;23(5):619-32. doi: 10.1101/gad.1760209. Epub 2009 Feb 24.
3
Gene expression and functional analysis of zebrafish larval fin fold regeneration.
Dev Biol. 2009 Jan 1;325(1):71-81. doi: 10.1016/j.ydbio.2008.09.028. Epub 2008 Oct 10.
4
Factorial microarray analysis of zebrafish retinal development.
Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):12909-14. doi: 10.1073/pnas.0806038105. Epub 2008 Aug 27.
5
Coronary development is regulated by ATP-dependent SWI/SNF chromatin remodeling component BAF180.
Dev Biol. 2008 Jul 15;319(2):258-66. doi: 10.1016/j.ydbio.2008.04.020. Epub 2008 Apr 24.
6
Gene delivery to muscle.
Curr Protoc Hum Genet. 2002 Feb;Chapter 13:Unit13.4. doi: 10.1002/0471142905.hg1304s31.
7
Dicer ablation affects antibody diversity and cell survival in the B lymphocyte lineage.
Cell. 2008 Mar 7;132(5):860-74. doi: 10.1016/j.cell.2008.02.020.
8
Endocardial Brg1 represses ADAMTS1 to maintain the microenvironment for myocardial morphogenesis.
Dev Cell. 2008 Feb;14(2):298-311. doi: 10.1016/j.devcel.2007.11.018.
9
Targeted deletion of Dicer in the heart leads to dilated cardiomyopathy and heart failure.
Proc Natl Acad Sci U S A. 2008 Feb 12;105(6):2111-6. doi: 10.1073/pnas.0710228105. Epub 2008 Feb 6.
10
Dicer-dependent pathways regulate chondrocyte proliferation and differentiation.
Proc Natl Acad Sci U S A. 2008 Feb 12;105(6):1949-54. doi: 10.1073/pnas.0707900105. Epub 2008 Jan 31.
Zotero 插件