Area de Nutrición y Bromatología, Facultad de Farmacia, Universidad de Sevilla, C/P. García González n degrees 2, Sevilla, Spain.
Talanta. 2010 Jun 15;81(4-5):1143-52. doi: 10.1016/j.talanta.2010.02.040. Epub 2010 Feb 19.
The amino acids present in foods and beverages affect the quality of these products and they play an important role in enology. Amino acids are consumed by yeasts as a source of nitrogen during alcoholic fermentation and are precursors of aroma compounds. In this review various chromatographic methodologies for the determination of amino acids are described, and specific applications for the analysis of amino acid content are discussed. Amino acids usually need to be derivatized to make them more detectable. Several derivatizing reagents have been employed for the determination of amino acids in enological applications, and each has its advantages and disadvantages.
食品和饮料中的氨基酸会影响这些产品的质量,并且在酿造学中起着重要作用。在酒精发酵过程中,氨基酸被酵母作为氮源消耗,并作为香气化合物的前体。在这篇综述中,描述了各种用于测定氨基酸的色谱方法学,并讨论了特定的氨基酸含量分析应用。通常需要对氨基酸进行衍生化以提高其检测灵敏度。已经使用了几种衍生化试剂来测定酿造学应用中的氨基酸,每种试剂都有其优点和缺点。