Kellermayer M, Jobst K, Szücs G
Cell Biol Int Rep. 1978 Jan;2(1):19-24. doi: 10.1016/0309-1651(78)90080-2.
Internuclear migration of tumour specific nuclear T antigen has been analysed in SV40-induced H-50 tumour and chick erythrocyte heterokaryons. Thirty hours after cell fusion the incorporated and enlarged erythrocyte nuclei were invariably T antigen-positive. When treated with colchicine at 10(-4) or 10(-7) M concentration, the erythrocyte nuclei incorporated into the heterokaryons did not swell and remained T antigen-negative. The results strongly suggest the involvement of a colchicine sensitive contractile protein matrix in the internuclear transport of T antigen and other proteins.
已在SV40诱导的H-50肿瘤和鸡红细胞异核体中分析了肿瘤特异性核T抗原的核内迁移。细胞融合30小时后,并入并增大的红细胞核始终呈T抗原阳性。当用浓度为10(-4)或10(-7)M的秋水仙碱处理时,并入异核体的红细胞核没有肿胀,并且仍为T抗原阴性。结果强烈表明,秋水仙碱敏感的收缩蛋白基质参与了T抗原和其他蛋白质的核间运输。