The nature of the proteins lost from isolated rat skeletal muscle during experimental damage.

Sodium dodecyl sulfate-polyacrylamide gel analysis of the incubation media surrounding isolated rat soleus muscles has been used to determine the nature of the proteins lost from skeletal muscle during damage. Following various forms of experimental trauma only selected proteins are lost to the medium when compared to the protein composition of the muscle cytosol. The proteins released do not appear to be released in a size dependent manner and their release is partly inhibited by an absence of calcium in the extracellular medium. alpha-Tocopherol entirely prevents the release of cytosolic enzymes induced by intracellular calcium overload.