State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, 220 Handan Road, Shanghai, People's Republic of China.
Vet Res Commun. 2010 Apr;34(4):335-46. doi: 10.1007/s11259-010-9360-y. Epub 2010 May 7.
Foot-and-mouth disease (FMD) is a highly contagious disease that afflicts cloven-hoofed animals. The etiological agent of FMD is foot-and-mouth disease virus (FMDV). The VP1 gene of FMDV is essential during the life cycle of the virus and plays a key role in the attachment of the virus to susceptible cells. We constructed a plasmid, pCWN11, that expresses siRNAs multiple-targeting the VP1 genes of FMDV. We evaluated the gene silencing efficiency of the plasmid using an enhanced green fluorescent protein (EGFP) reporter system in BHK-21 cells. The antiviral potential of the plasmid in BHK-21 cells and suckling mice were investigated. The results indicate that cotransfection of pCWN11 with any one of three serotypes VP1-EGFP plasmids resulted in a reduction in the EGFP signal relative to the control. Moreover, the antiviral potential induced by pCWN11 was evident during challenge with one FMDV isolate of either serotype O (HKN/2002) or serotype Asia I (YNBS/58), and the inhibition extended to almost 40 h. Furthermore, subcutaneous injection of pCWN11 in the neck made suckling mice significantly less susceptible to FMDV serotype O and Asia I.
口蹄疫(FMD)是一种高度传染性疾病,影响偶蹄类动物。FMD 的病原体是口蹄疫病毒(FMDV)。FMDV 的 VP1 基因在病毒的生命周期中至关重要,在病毒附着于易感细胞中发挥关键作用。我们构建了一个表达针对 FMDV VP1 基因的多个 siRNA 的质粒 pCWN11。我们使用 BHK-21 细胞中的增强型绿色荧光蛋白(EGFP)报告系统评估了质粒的基因沉默效率。研究了质粒在 BHK-21 细胞和乳鼠中的抗病毒潜力。结果表明,pCWN11 与三种血清型 VP1-EGFP 质粒中的任何一种共转染都会导致 EGFP 信号相对于对照降低。此外,pCWN11 诱导的抗病毒潜力在与 O 型(HKN/2002)或亚洲 I 型(YNBS/58)的 FMDV 分离株的攻毒中显而易见,抑制作用持续近 40 小时。此外,颈部皮下注射 pCWN11 使乳鼠对 O 型和亚洲 I 型 FMDV 的易感性显著降低。