重组转座因子-TRAX 复合物与核酸的结合分析。
Analysis of nucleic acid binding by a recombinant translin-trax complex.
机构信息
Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas, Austin, TX 78712, USA.
出版信息
Biochem Biophys Res Commun. 2010 Jun 4;396(3):709-13. doi: 10.1016/j.bbrc.2010.04.166. Epub 2010 May 5.
Translin is a highly conserved mammalian RNA and DNA-binding protein involved in DNA recombination and RNA trafficking. Crystal structures of mouse and human translin have been solved, but do not provide information about nucleic acid binding or recognition. Translin has a partner protein, translin-associated factor x (trax), which is believed to regulate translin's subcellular locale and affinity for certain RNA and DNA sequences. Here we present a comparative study of recombinant translin and translin-trax complex binding to specific RNA and DNA sequences. It was observed that translin preferentially binds to G-rich RNA sequences whereas translin-trax preferentially binds G-rich DNA sequences. Translin can bind mRNA sequences with sub-micromolar K(d) values, and the complex with trax can bind G-rich DNA with similar affinity. We conclude that trax acts to regulate translin's RNA and DNA binding affinities as part of a cellular RNA trafficking mechanism.
转译因子是一种高度保守的哺乳动物 RNA 和 DNA 结合蛋白，参与 DNA 重组和 RNA 转运。已经解析了小鼠和人类转译因子的晶体结构，但这些结构并不能提供关于核酸结合或识别的信息。转译因子有一个伴侣蛋白，即转译因子相关因子 x（trax），它被认为可以调节转译因子的亚细胞定位和对某些 RNA 和 DNA 序列的亲和力。在这里，我们对重组转译因子和转译因子-相关因子 x 复合物与特定 RNA 和 DNA 序列的结合进行了比较研究。结果表明，转译因子优先结合富含 G 的 RNA 序列，而转译因子-相关因子 x 复合物优先结合富含 G 的 DNA 序列。转译因子可以与 mRNA 序列以亚毫摩尔 K(d)值结合，而与 trax 的复合物可以以类似的亲和力与富含 G 的 DNA 结合。我们的结论是，trax 作为细胞 RNA 转运机制的一部分，调节转译因子的 RNA 和 DNA 结合亲和力。
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