Veterinary Teaching Hospital, Laboratory of Spermatology, University of Extremadura Avd de la Universidad s/n Cáceres Spain.
Theriogenology. 2010 Aug;74(3):458-65. doi: 10.1016/j.theriogenology.2010.02.029. Epub 2010 May 10.
In order to evaluate to what extent the changes that occur during cryopreservation involve the mitochondrial permeability transition pore (PT-pore), a specific inhibitor was used during the cryopreservation process of stallion spermatozoa. Four ejaculates from each of 7 stallions were frozen using a standard protocol. Before freezing, each ejaculate was split into three subsamples. The first was supplemented with 2.5 microM bongkrekic acid (BA) and the second with 5 microM BA. The third subsample served as control. The BA significantly reduced the percentage of spermatozoa depicting active caspases after thawing, reduced the percentage of spermatozoa with increased membrane permeability, and increased the mitochondrial membrane potential of thawed sperm. Sperm motility was reduced as a result of the treatment. It is concluded that the mitochondrial pathway of apoptosis seems to be an important factor involved in the sublethal damage that equine spermatozoa experience after freezing and thawing, and that sperm motility in the equine species is largely dependent on mitochondrial ATP produced by oxidative phosphorylation.
为了评估在冷冻过程中发生的变化在何种程度上涉及线粒体通透性转换孔（PT 孔），在马精子的冷冻保存过程中使用了一种特定的抑制剂。从 7 匹种马的每匹中采集了 4 份精液，使用标准方案进行冷冻。在冷冻之前，每份精液被分为三个亚样本。第一个亚样本中添加了 2.5μM 绷克雷酸（BA），第二个亚样本中添加了 5μM BA。第三个亚样本作为对照。BA 显著降低了解冻后显示活性半胱天冬酶的精子百分比，降低了膜通透性增加的精子百分比，并增加了解冻精子的线粒体膜电位。由于处理，精子运动能力降低。研究结论认为，细胞凋亡的线粒体途径似乎是冷冻和解冻后马精子经历的亚致死损伤的一个重要因素，而马属动物的精子运动能力在很大程度上依赖于由氧化磷酸化产生的线粒体 ATP。
