Department of Biological Sciences, Inje University, Kyungnam, Kimhae 621-749, Republic of Korea.
Fungal Genet Biol. 2010 Aug;47(8):713-20. doi: 10.1016/j.fgb.2010.04.005. Epub 2010 May 7.
Allomyces macrogynus, a true fungus, produces zoosporangia which discharge uninucleate zoospores after cytoplasmic cleavage. Binucleate zoosporangia of A. macrogynus were induced and examined to understand the basic principles of cytokinesis associated with the multinucleate zoosporangia. Development of cleavage membranes was visualized by constructing three dimensional models based on electron micrographs and confocal images. Cleavage membranes on the cleavage plane showed asymmetric ingression from the cortex, but cleavage of cytoplasm was completed by the fusion of cleavage membranes with plasma membrane. Also, the position of the cleavage plane was continuously rotated until settled at the last stage. These studies suggest that the positions of the numerous cleavage planes within a multinucleate zoosporangium are continuously adjusted during development of cleavage membranes. The final settlement of cleavage planes would define the exact boundary of cleavage planes and the expansion of cleavage membranes toward the boundary could complete the cleavage of cytoplasm.
大团囊菌是一种真正的真菌，它产生游动孢子囊，游动孢子囊在细胞质分裂后释放出单核游动孢子。为了了解与多核游动孢子囊相关的胞质分裂的基本原理，我们诱导并检查了大团囊菌的双核游动孢子囊。通过构建基于电子显微镜和共聚焦图像的三维模型，观察到分裂膜的发育。在分裂面上的分裂膜从皮层不对称内陷，但细胞质的分裂是通过分裂膜与质膜的融合完成的。此外，分裂面的位置不断旋转，直到最后阶段固定。这些研究表明，在多核游动孢子囊的众多分裂面的位置在分裂膜的发育过程中不断调整。分裂面的最终固定将确定分裂面的确切边界，并且分裂膜向边界的扩展可以完成细胞质的分裂。
