多酚苹果提取物：原料和生产方法对 Caco-2 细胞抗氧化活性和 DNA 损伤减少的影响。
Polyphenolic apple extracts: effects of raw material and production method on antioxidant effectiveness and reduction of DNA damage in Caco-2 cells.
机构信息
Department of Chemistry, Division of Food Chemistry & Toxicology, University of Kaiserslautern, Erwin Schroedinger Strasse 52, 67663 Kaiserslautern, Germany.
出版信息
J Agric Food Chem. 2010 Jun 9;58(11):6636-42. doi: 10.1021/jf904150x.
A diet rich in fruits and vegetables is commonly perceived to be associated with reduced cancer risk, attributed to its high content of polyphenols. As apples represent a major polyphenol source in Western countries, we studied differentially produced extracts (1-100 microg/mL): two from different apple juices (AEs), one from pomace (APE), and one peel extract (PE) on their potential to reduce DNA oxidation damage and induce antioxidant defense in Caco-2 cells. Additionally, we measured direct antioxidant capacity (TEAC/ORAC) of the extracts. Quercetin-rich PE and APE most effectively diminished DNA damage and ROS level after 24 h incubation (PE > APE), whereas the AEs were only moderately effective. GPx activity was diminished for all extracts, with AEs > APE > PE. Direct antioxidant activity decreased in the order AEs > PE > APE, displaying no significant correlation with cellular markers. In conclusion, apple phenolics at low, nutritionally relevant concentrations may protect intestinal cells from ROS-induced DNA damage, mediated by cellular defense mechanisms rather than by antioxidant activity.
富含水果和蔬菜的饮食通常被认为与降低癌症风险有关，这归因于其富含多酚。由于苹果是西方国家多酚的主要来源之一，我们研究了不同制备提取物（1-100 微克/毫升）：两种来自不同的苹果汁（AE），一种来自苹果渣（APE）和一种果皮提取物（PE），以评估它们在降低 Caco-2 细胞 DNA 氧化损伤和诱导抗氧化防御方面的潜力。此外，我们还测量了提取物的直接抗氧化能力（TEAC/ORAC）。富-Quercetin 的 PE 和 APE 在 24 小时孵育后最有效地减少 DNA 损伤和 ROS 水平（PE > APE），而 AE 则只有适度的效果。所有提取物均降低了 GPx 活性，AE > APE > PE。直接抗氧化活性的顺序为 AE > PE > APE，与细胞标志物无显著相关性。总之，在低浓度、营养相关浓度下，苹果酚类物质可能通过细胞防御机制而不是抗氧化活性来保护肠道细胞免受 ROS 诱导的 DNA 损伤。
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