Department of Physiology, Dartmouth Medical School, One Medical Center Drive, Lebanon, NH 03756, USA.
Am J Reprod Immunol. 2010 Sep;64(3):197-211. doi: 10.1111/j.1600-0897.2010.00850.x.
communication between uterine epithelial cells and the underlying stromal fibroblasts is critical for proper endometrial function. Stromal fibroblast-derived growth factors have been shown to regulate epithelial immune functions. The purpose of this study was to determine whether keratinocyte growth factor (KGF) regulates uterine epithelial cell chemokine and antimicrobial secretion.
uterine epithelial cells were isolated from Balb/c mice and cultured in either 96-well plates or transwell inserts. Epithelial cells were treated with KGF, epidermal growth factor (EGF), or hepatocyte growth factor (HGF). Macrophage inflammatory protein 3α (MIP3α) and keratinocyte-derived chemokine (KC) levels were measured by ELISA.
keratinocyte growth factor stimulated the secretion of MIP3α and KC. The effects on MIP3α by KGF were specific because EGF and HGF had no effect. In contrast, KGF, EGF, and HGF had similar effects on KC. Furthermore, KGF administered to the apical side of epithelial cells had no effect on MIP3α or KC secretion, indicating that the KGF receptor is located on the basolateral surface of uterine epithelial cells.
we demonstrate that KGF plays a role in uterine epithelial cell secretion of MIP3α and KC, key immune mediators involved in the protection of mucosal surfaces in the female reproductive tract.
子宫上皮细胞与下方基质成纤维细胞之间的通讯对于子宫内膜的正常功能至关重要。已经表明基质成纤维细胞衍生的生长因子可调节上皮免疫功能。本研究的目的是确定角质细胞生长因子（KGF）是否调节子宫上皮细胞趋化因子和抗菌肽的分泌。
从小鼠的子宫中分离出上皮细胞，并在 96 孔板或 Transwell 插入物中进行培养。用 KGF、表皮生长因子（EGF）或肝细胞生长因子（HGF）处理上皮细胞。通过 ELISA 测量巨噬细胞炎性蛋白 3α（MIP3α）和角质细胞衍生的趋化因子（KC）的水平。
角质细胞生长因子刺激 MIP3α 和 KC 的分泌。KGF 对 MIP3α 的作用是特异性的，因为 EGF 和 HGF 没有作用。相比之下，KGF、EGF 和 HGF 对 KC 具有相似的作用。此外，在细胞的顶侧施加 KGF 对 MIP3α 或 KC 的分泌没有影响，这表明 KGF 受体位于子宫上皮细胞的基底外侧表面。
我们证明 KGF 在子宫上皮细胞分泌 MIP3α 和 KC 中起作用，MIP3α 和 KC 是参与保护女性生殖道黏膜表面的关键免疫介质。
