Krylova E D, Gorb T E, Romaniuk L V, Tovkach F I
Mikrobiol Z. 2009 Sep-Oct;71(5):72-8.
The method of quantitative determination of bacteriocinogenicity in Erwinia carotovora dissociants has been suggested. It is based on the application of indicator bacterial mutants that are resistant to nalidixic acid. It has been revealed that population dissociation destabilizes a defective lysogeny of pectolytic Erwinia. In particular, a decrease of cell indicator survivability due to an increase of active bacteriocins yield has been found under lysogenic induction of defective prophages. The reverse dependence between the indicator cell survivability caused by dissociants bacteriocins induction and the reaction of hypersensitivity on leaves of the resistant plant Nicotiana tabacum, has been revealed. Similar dependence has been determined between dissociation and activity of pectate lyase. It has been hypothesized, that viable erwiniophages, being involved in the process of lysogenicity and induction, could play the role of 'switches' of bacterial phenotype raising adaptive phytopathogene reactions. The paper is presented in Russian. K e y w o r d s: Erwinia carotovora, defective lysogeny, population dissociation, reaction of hypersensitivity, activity of pectate lyase.
已提出测定胡萝卜软腐欧文氏菌解离体中产生细菌素能力的定量方法。该方法基于使用对萘啶酸具有抗性的指示细菌突变体。已发现群体解离会破坏果胶分解欧文氏菌的缺陷溶原性。特别是，在缺陷原噬菌体的溶原诱导下，由于活性细菌素产量的增加，发现指示细胞的存活率降低。已揭示解离体细菌素诱导引起的指示细胞存活率与抗性植物烟草叶片过敏反应之间存在反向依赖关系。在解离与果胶酸裂解酶活性之间也确定了类似的依赖关系。据推测，参与溶原性和诱导过程的活欧文氏噬菌体可能起到“开关”的作用，引发适应性植物病原菌反应。本文用俄语发表。关键词：胡萝卜软腐欧文氏菌、缺陷溶原性、群体解离、过敏反应、果胶酸裂解酶活性
