灵芝甲醇提取物对小鼠急性瘙痒相关反应的抑制作用。

Inhibitory effect of methanol extract of Ganoderma lucidum on acute itch-associated responses in mice.

机构信息

Department of Applied Pharmacology, University of Toyama, 2630 Sugitani, Toyama 930-0914, Japan.

出版信息

Biol Pharm Bull. 2010;33(5):909-11. doi: 10.1248/bpb.33.909.

DOI:10.1248/bpb.33.909

PMID:20460776

Abstract

In this study, the antipruritic effect of the methanol extract of Ganoderma lucidum (MEGL) was studied in mice. Oral administration of MEGL (10-1000 mg/kg) produced a dose-dependent inhibition of scratching, an itch-related response, induced by intradermal 5-hydroxytryptamine (5-HT) (100 nmol/site), alpha-methyl-5-HT (100 nmol/site), and proteinase-activated receptor-2 (PAR(2))-activating peptide SLIGRL-NH(2) (50 nmol/site). However, MEGL (100-1000 mg/kg) did not inhibit the scratching induced by histamine (100 nmol/site), substance P (100 nmol/site), and compound 48/80 (10 microg/site). These results raise the possibility that MEGL is effective against pruritus mediated by proteinases and 5-HT and that primary afferents expressing PAR(2) and 5-HT(2A) receptors are the sites of its action.

摘要

在这项研究中，研究了灵芝甲醇提取物（MEGL）对小鼠的止痒作用。MEGL（10-1000mg/kg）经口给予可剂量依赖性抑制 5-羟色胺（5-HT）（100nmol/site）、α-甲基-5-HT（100nmol/site）和蛋白酶激活受体-2（PAR（2））激活肽 SLIGRL-NH（2）（50nmol/site）诱导的搔抓，这是一种与瘙痒相关的反应。然而，MEGL（100-1000mg/kg）不能抑制组胺（100nmol/site）、P 物质（100nmol/site）和化合物 48/80（10μg/site）诱导的搔抓。这些结果提示 MEGL 可能对蛋白酶和 5-HT 介导的瘙痒有效，并且表达 PAR（2）和 5-HT（2A）受体的初级传入神经是其作用部位。