Nanoprobes, Inc., Yaphank, NY 11980, USA.
Phys Med Biol. 2010 Jun 7;55(11):3045-59. doi: 10.1088/0031-9155/55/11/004. Epub 2010 May 12.
The purpose of this study is to test the hypothesis that gold nanoparticle (AuNP, nanogold)-enhanced radiation therapy (nanogold radiation therapy, NRT) is efficacious when treating the radiation resistant and highly aggressive mouse head and neck squamous cell carcinoma model, SCCVII, and to identify parameters influencing the efficacy of NRT. Subcutaneous (sc) SCCVII leg tumors in mice were irradiated with x-rays at the Brookhaven National Laboratory (BNL) National Synchrotron Light Source (NSLS) with and without prior intravenous (iv) administration of AuNPs. Variables studied included radiation dose, beam energy, temporal fractionation and hyperthermia. AuNP-mediated NRT was shown to be effective for the sc SCCVII model. AuNPs were more effective at 42 Gy than at 30 Gy (both at 68 keV median beam energy) compared to controls without gold. Similarly, at 157 keV median beam energy, 50.6 Gy NRT was more effective than 44 Gy NRT. At the same radiation dose ( approximately 42 Gy), 68 keV was more effective than 157 keV. Hyperthermia and radiation therapy (RT) were synergistic and AuNPs enhanced this synergy, thereby further reducing TCD50 s (tumor control dose 50%) and increasing long-term survivals. It is concluded that gold nanoparticles enhance the radiation therapy of a radioresistant mouse squamous cell carcinoma. The data show that radiation dose, energy and hyperthermia influence efficacy and better define the potential utility of gold nanoparticles for cancer x-ray therapy.
本研究旨在验证金纳米粒子（AuNP，纳米金）增强放射疗法（纳米金放射疗法，NRT）在治疗辐射抗性和高度侵袭性的小鼠头颈部鳞状细胞癌模型 SCCVII 时是否有效的假设，并确定影响 NRT 疗效的参数。在布鲁克海文国家实验室（BNL）国家同步辐射光源（NSLS）用 X 射线辐照小鼠皮下（sc）SCCVII 腿部肿瘤，同时或不事先静脉内（iv）给予 AuNPs。研究的变量包括辐射剂量、束能量、时间分割和热疗。AuNP 介导的 NRT 对 sc SCCVII 模型有效。与没有金的对照组相比，AuNPs 在 42Gy 时比在 30Gy（均在 68keV 中值束能量下）更有效。同样，在 157keV 中值束能量下，50.6Gy 的 NRT 比 44Gy 的 NRT 更有效。在相同的辐射剂量（约 42Gy）下，68keV 比 157keV 更有效。热疗和放射治疗（RT）具有协同作用，AuNPs 增强了这种协同作用，从而进一步降低 TCD50s（肿瘤控制剂量 50%）并提高长期存活率。结论是，金纳米粒子增强了一种辐射抗性小鼠鳞状细胞癌的放射治疗。数据表明，辐射剂量、能量和热疗影响疗效，并更好地定义了金纳米粒子用于癌症 X 射线治疗的潜在效用。
