抗生素对原核生物与真核生物解码位点的选择性。
Antibiotic selectivity for prokaryotic vs. eukaryotic decoding sites.
机构信息
Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA 92093-0358, USA.
出版信息
Chem Commun (Camb). 2010 Aug 14;46(30):5542-4. doi: 10.1039/c0cc00423e. Epub 2010 May 13.
Abstract
A FRET assembly reports antibiotic affinities to two different RNA targets. A binder was labeled with a fluorophore that acts both as an acceptor for the emissive nucleoside on the bacterial A-site and a donor fluorophore for the terminally-labeled human A-site. Unlabeled drugs were used to dissociate the labeled antibiotic.
摘要
FRET 组装报告抗生素对两种不同 RNA 靶标的亲和力。配体用荧光团标记，该荧光团既可以作为细菌 A 位上发射核苷的受体，也可以作为末端标记的人 A 位的供体荧光团。未标记的药物用于解离标记的抗生素。
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