Perić Aleksandar, Vojvodić Danilo, Radulović Vesna, Miljanović Olivera
Clinic for Otorhinolaryngology, Department of Rhinology, Military Medical Academy, Belgrade, Serbia.
Kulak Burun Bogaz Ihtis Derg. 2010 May-Jun;20(3):111-7.
The aim of this study was to compare the cytokine levels of subjects with nasal polyps (NP) and comorbid asthma and NP patients without asthma.
Thirty patients with NP (15 asthmatic and 15 nonasthmatic) were included in this prospective study. Nasal secretion samples were collected from the nasal cavities of all subjects. The levels of eleven cytokines (TNF-alpha, TNF-beta, IL-1beta, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12, and IFN-gamma) were measured using flow cytometric method.
The concentrations of Th2 cytokines IL-5, IL-6 and IL-10 were significantly higher in patients with NP and asthma compared with subjects with NP without asthma. IFN-gamma, IL-4, IL-6 and IL-10 levels were found significantly higher in allergic patients with NP and asthma compared with those without asthma. In nonallergic patients with NP and asthma, the concentrations of TNF-alpha, IL-5 and IL-6 were significantly higher than in nonallergic patients with NP without asthma.
Our results showed that the presence of Th2 cytokines, especially IL-5 and IL-6 in patients with NP and asthma is a prominent feature that relates to increased eosinophilic inflammation. We also found significant influence of asthma and allergy on the cytokine profiles in nasal secretions of patients with NP.
本研究旨在比较鼻息肉（NP）合并哮喘患者与无哮喘的NP患者的细胞因子水平。
本前瞻性研究纳入了30例NP患者（15例哮喘患者和15例非哮喘患者）。从所有受试者的鼻腔收集鼻分泌物样本。使用流式细胞术测量11种细胞因子（肿瘤坏死因子-α、肿瘤坏死因子-β、白细胞介素-1β、白细胞介素-2、白细胞介素-4、白细胞介素-5、白细胞介素-6、白细胞介素-8、白细胞介素-10、白细胞介素-12和干扰素-γ）的水平。
与无哮喘的NP患者相比，NP合并哮喘患者中Th2细胞因子白细胞介素-5、白细胞介素-6和白细胞介素-10的浓度显著更高。与无哮喘的患者相比，NP合并哮喘的过敏患者中干扰素-γ、白细胞介素-4、白细胞介素-6和白细胞介素-10水平显著更高。在非过敏的NP合并哮喘患者中，肿瘤坏死因子-α、白细胞介素-5和白细胞介素-6的浓度显著高于非过敏的无哮喘NP患者。
我们的结果表明，NP合并哮喘患者中Th2细胞因子的存在，尤其是白细胞介素-5和白细胞介素-6，是与嗜酸性粒细胞炎症增加相关的一个突出特征。我们还发现哮喘和过敏对NP患者鼻分泌物中的细胞因子谱有显著影响。
