Yan Rongjin, Lynn N Scott, Kingry Luke C, Slayden Richard A, Dandy David S, Lear Kevin L
Colorado State University.
Biomed Sci Instrum. 2010;46:45-50.
A label-free optical waveguide immunosensor was designed, fabricated and tested. Different from other popular resonance-based biosensors, such as surface-plasmon-resonance (SPR) or ring/disk resonance biosensors, the local evanescent array coupled (LEAC) biosensor relies on a local evanescent field shift mechanism and can be readily manufactured using trailing-edge integrated-circuit technology with chip-scale microfluidics technology to provide very low cost. The anticipated final form of the sensor technology will require no external equipment enabling disposable use for point-of-care disease detection in non-traditional health-care settings. The local detection ability enables the LEAC biosensor the capability to detect and identify multiple analytes on the same waveguide. On-chip detection is accomplished by integrating buried polysilicon detector arrays under a silicon nitride waveguide. Observations of antibody-antigen interactions using the LEAC biosensor are reported.
设计、制造并测试了一种无标记光波导免疫传感器。与其他流行的基于共振的生物传感器不同,如表面等离子体共振(SPR)或环形/盘形共振生物传感器,局部倏逝场阵列耦合(LEAC)生物传感器依赖于局部倏逝场位移机制,并且可以使用后缘集成电路技术和芯片级微流控技术轻松制造,从而实现非常低成本。传感器技术的预期最终形式将无需外部设备,从而能够在非传统医疗环境中一次性用于即时护理疾病检测。局部检测能力使LEAC生物传感器能够在同一波导上检测和识别多种分析物。芯片上的检测是通过在氮化硅波导下方集成掩埋多晶硅探测器阵列来完成的。本文报道了使用LEAC生物传感器对抗体-抗原相互作用的观察结果。