比较抗 TNF-α 药物对葡萄膜炎实验模型的急性作用。

Comparison of the acute effects of anti-TNF-alpha drugs on a uveitis experimental model.

机构信息

Fundación Oftalmológica del Mediterráneo, Valencia, Spain.

出版信息

Ocul Immunol Inflamm. 2010 Jun;18(3):208-15. doi: 10.3109/09273940903521964.

DOI:10.3109/09273940903521964

PMID:20482400

Abstract

PURPOSE

To compare histopathological and biochemical effects of the anti-TNF-alpha drugs adalimumab and infliximab in a uveitis experimental model.

METHODS

Histopathological evaluation was performed 24 h after endotoxin (200 microg into the footpad) and drug administration, as well as biochemical analysis of oxidative stress-related markers in the aqueous humor.

RESULTS

Severe inflammation was found in rat anterior chamber of the eye 24 h after endotoxin. Only infliximab administration partially prevented the endotoxin-induced disruption of the blood-aqueous barrier, as well as the increase in Rantes and MCP-1 concentration in aqueous humor. Both drugs ameliorated the histopathological score after endotoxin. Biochemical analysis revealed that both drugs protected against endotoxin-induced oxidative stress, restoring all markers to control levels, except infliximab, which failed to restore GSH concentration.

CONCLUSIONS

Both anti-TNF-alpha drugs were effective in reducing histopathological inflammation but their mechanism of action appears to be different.

摘要

目的

比较抗 TNF-α 药物阿达木单抗和英夫利昔单抗在葡萄膜炎实验模型中的组织病理学和生化效应。

方法

在给予内毒素（200μg 至足底）和药物后 24 小时进行组织病理学评估，并分析房水氧化应激相关标志物的生化分析。

结果

内毒素后 24 小时，大鼠眼前房出现严重炎症。只有英夫利昔单抗给药部分预防了内毒素引起的血房水屏障破坏，以及房水中 Rantes 和 MCP-1 浓度的增加。两种药物均改善了内毒素后的组织病理学评分。生化分析表明，两种药物均能对抗内毒素诱导的氧化应激，除英夫利昔单抗外，所有标志物均恢复至对照水平，而英夫利昔单抗未能恢复 GSH 浓度。