The Harrington Department of Bioengineering, Center for Interventional Biomaterials, Arizona State University, Tempe, AZ 85287-9709, USA.
J Biomater Sci Polym Ed. 2010;21(6-7):913-26. doi: 10.1163/156856209X451323.
A thermosensitive, bioresorbable and in situ gelling co-polymer, poly(N-isopropylacrylamide-co-dimethyl-gamma-butyrolactone acrylate-co-acrylic acid), was synthesized by radical co-polymerization with varying dimethyl-gamma-butyrolactone acrylate (DBA) content. The materials properties were characterized using differential scanning calorimetry, gel-permeation chromatography in conjunction with static light scattering, Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR) and acid titration. The initial lower critical solution temperature (LCST) of the synthesized co-polymer is between room temperature and body temperature. With the increase of DBA content, the LCST decreases, but then increases after the ring-opening hydrolysis of the DBA side-group. The FT-IR and NMR spectra show the co-polymerization of three monomers, as well as the hydrolysis-dependent ring-opening of the DBA side-group. The addition of acrylic acid increases the initial LCST and accelerates the degradation rate of the co-polymer. An indirect cytotoxicity test indicated that this co-polymer has relatively low cytotoxicity as seen with 3T3 fibroblast cells.
一种温敏、可生物降解和原位凝胶化的共聚物，聚（N-异丙基丙烯酰胺-co-二甲基-γ-丁内酯丙烯酸酯-co-丙烯酸），通过自由基共聚反应合成，其中二甲基-γ-丁内酯丙烯酸酯（DBA）的含量不同。使用差示扫描量热法、凝胶渗透色谱法与静态光散射法、傅里叶变换红外光谱（FT-IR）、核磁共振（NMR）和酸滴定法对材料性能进行了表征。合成共聚物的初始低临界溶液温度（LCST）在室温到体温之间。随着 DBA 含量的增加，LCST 降低，但随后在 DBA 侧基的开环水解后又升高。FT-IR 和 NMR 谱表明三种单体的共聚，以及 DBA 侧基的水解依赖性开环。丙烯酸的加入增加了初始 LCST 并加速了共聚物的降解速率。间接细胞毒性试验表明，这种共聚物的细胞毒性相对较低，与 3T3 成纤维细胞相似。
