大鼠脑内三种烯醇化酶同工酶从单细胞胚胎到成年脑的发育概况测定

Developmental profile of three enolase isozymes in rat brain determination from one-cell embryo to adult brain.

作者信息

Kato K, Suzuki F, Watanabe T, Semba R, Keino H

机构信息

Department of Biochemistry, Institute for Developmental Research, Aichi Prefectural Colony, Kasugai, Aichi, Japan.

出版信息

Neurochem Int. 1984;6(1):51-4. doi: 10.1016/0197-0186(84)90025-1.

DOI:10.1016/0197-0186(84)90025-1

PMID:20488019

Abstract

Levels of three enolase isozymes (??, ?? and ??) were determined in rat tissues from one-cell embryo to adult brain with a sensitive enzyme immunoassay system. Each embryo of the early stage (gestational age, 0-3 days) contained about 5 x 10(?17) mol of ?? enolase. The nervous system-specific ?? and ?? enolases would be detected in the embryos of 6-8 days, which contain no histologically recognizable neurones. The 8-day embryos contained 4.3 x 10(?17) and 3.4 x 10(?16) mol of ?? and ?? enolases. Amounts of all the three enolases were increased with growth of the embryo. The nervous system-specific enolases (?? and ??) in the brain kept increasing until 1-2 months of postnatal age, whereas the ?? enolase level in the brain was relatively constant after the 15-day embryo through the adult rat.

摘要

采用灵敏的酶免疫分析系统，测定了从单细胞胚胎到成年大鼠脑组织中三种烯醇化酶同工酶（α、β和γ）的水平。早期胚胎（妊娠0 - 3天）每个胚胎约含5×10⁻¹⁷摩尔的α烯醇化酶。在6 - 8天的胚胎中可检测到神经系统特异性的β和γ烯醇化酶，此时尚无组织学上可识别的神经元。8天的胚胎含有4.3×10⁻¹⁷摩尔的β烯醇化酶和3.4×10⁻¹⁶摩尔的γ烯醇化酶。随着胚胎的生长，三种烯醇化酶的含量均增加。脑中神经系统特异性烯醇化酶（β和γ）一直增加到出生后1 - 2个月，而从胚胎15天到成年大鼠，脑中α烯醇化酶水平相对恒定。