Institut für Mikrobiologie und Molekularbiologie der JLU Giessen, Giessen, Germany.
FEBS Lett. 2010 Jul 2;584(13):2791-5. doi: 10.1016/j.febslet.2010.05.013. Epub 2010 May 17.
We studied the cellular localization of the archaeal exosome, an RNA-processing protein complex containing orthologs of the eukaryotic proteins Rrp41, Rrp42, Rrp4 and Csl4, and an archaea-specific subunit annotated as DnaG. Fractionation of cell-free extracts of Sulfolobus solfataricus in sucrose density gradients revealed that DnaG and the active-site comprising subunit Rrp41 are enriched together with surface layer proteins in a yellow colored ring, implicating that the exosome is membrane-bound. In accordance with this assumption, DnaG and Rrp41 were detected at the periphery of the cell by immunofluorescence microscopy. Our finding suggests that RNA processing in Archaea is spatially organized.
我们研究了古菌核酶的细胞定位，这是一种 RNA 加工蛋白复合物，含有真核生物蛋白 Rrp41、Rrp42、Rrp4 和 Csl4 的同源物，以及一个被注释为 DnaG 的古菌特异性亚基。在蔗糖密度梯度中对 Sulfolobus solfataricus 无细胞提取物进行分级分离，结果表明 DnaG 和含有活性位点的亚基 Rrp41 与表面层蛋白一起富集在一个黄色环中，这表明核酶是膜结合的。根据这一假设，通过免疫荧光显微镜在细胞周围检测到 DnaG 和 Rrp41。我们的发现表明，古菌的 RNA 加工是空间组织的。
