[锂-匹罗卡品诱导癫痫发作后成年大鼠齿状回中白细胞介素-1信使核糖核酸的表达]
[Expression of IL-1 mRNA in the dentate gyrus of adult rats following lithium-pilocarione-induced seizures].
作者信息
Zhang Shi-jun, Li Xiao-wei, Wang Ying, Wei Dong, Jiang Wen
机构信息
Department of Neurology, Xijing Hospital, The Fourth Military Medical University, Xi'an, China.
出版信息
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2010 Mar;26(3):288-90.
AIM
To examine the expression of IL-1beta, IL-1ra and IL-1R mRNA in the dentate gyrus of adult rats after lithium-pilocarpine (Li-PILO)-induced seizures.
METHODS
Seizure epilepticus (SE) was induced using Li-PILO intraperitoneally (i.p.) injection. Lithium chloride was injected 18-20 h prior to PILO. The rats in the control group were given physiological saline instead of pilocarpine. We observed the behavior of rats and examined the expression of cytokines mRNA in dentate gyrus by RT-PCR.
RESULTS
Rats had seizure epilepticus in 30 min after administrated of pilocarpine. Cytokines (IL-1beta, IL-1ra and IL-1R) began to increase significantly (P<0.05) at 1 h after SE, and the peak was at 6-12 h. Expression of all cytokines declined on 48 h after SE.
CONCLUSION
Our findings suggested that SE lead to increased expression of IL-1, IL-1ra and IL-1R1 after lithium-pilocarpine-induced seizures, indicating that cytokines involved in SE and brain impairment after seizure.
目的
研究锂-匹罗卡品（Li-PILO）诱导癫痫发作后成年大鼠齿状回中白细胞介素-1β（IL-1β）、白细胞介素-1受体拮抗剂（IL-1ra）和白细胞介素-1受体（IL-1R）mRNA的表达。
方法
采用腹腔注射Li-PILO诱导癫痫持续状态（SE）。在注射匹罗卡品前18 - 20小时注射氯化锂。对照组大鼠注射生理盐水而非匹罗卡品。观察大鼠行为，并通过逆转录聚合酶链反应（RT-PCR）检测齿状回中细胞因子mRNA的表达。
结果
注射匹罗卡品后30分钟大鼠出现癫痫持续状态。癫痫持续状态后1小时细胞因子（IL-1β、IL-1ra和IL-1R）开始显著增加（P<0.05），峰值出现在6 - 12小时。癫痫持续状态后48小时所有细胞因子的表达均下降。
结论
我们的研究结果表明，锂-匹罗卡品诱导癫痫发作后，癫痫持续状态导致IL-1、IL-1ra和IL-1R1表达增加，表明细胞因子参与了癫痫持续状态及发作后脑损伤。
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