Laboratory of Germ Cell Biology, Qingdao Agricultural University, Qingdao, China.
Histochem Cell Biol. 2010 Jul;134(1):75-82. doi: 10.1007/s00418-010-0708-8. Epub 2010 May 22.
Folliculogenesis is a critical process during mammalian development, but little is known about the mechanisms underlying primordial follicular formation, growth, and activation. The objective of this study was to assess the effect of continuous insulin exposure on folliculogenesis in 16.5 dpc and 3 dpp mouse ovaries in vitro. The results demonstrated that 5 microg/ml insulin promotes the phosphorylation of Akt and the primordial follicular assembly and activation process via the InsR/PI3K/Akt signaling pathway in the 16.5 dpc fetal mouse ovaries cultured in vitro. However, the presence of 5 microg/ml insulin represses the phosphorylation of Akt and the follicular assembly and activation process in the 3 dpp mouse ovaries cultured in vitro. Furthermore, in vitro cultures of 3 dpp mouse ovaries with insulin can repress the expression of Pten. In conclusion, folliculogenesis was stage-specifically regulated by insulin via the Akt signaling pathway in vitro.
卵泡发生是哺乳动物发育过程中的一个关键过程，但对于原始卵泡形成、生长和激活的机制知之甚少。本研究旨在评估持续胰岛素暴露对体外培养的 16.5 dpc 和 3 dpp 小鼠卵巢卵泡发生的影响。结果表明，5μg/ml 胰岛素通过 InsR/PI3K/Akt 信号通路促进 Akt 的磷酸化以及原始卵泡的组装和激活过程，在体外培养的 16.5 dpc 胎鼠卵巢中。然而，在体外培养的 3 dpp 小鼠卵巢中，5μg/ml 胰岛素抑制 Akt 的磷酸化以及卵泡的组装和激活过程。此外，胰岛素体外培养 3 dpp 小鼠卵巢可抑制 Pten 的表达。总之，胰岛素通过 Akt 信号通路在体外对卵泡发生进行了阶段特异性调节。
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