鉴定小檗碱为西尼罗河病毒抑制剂。

Identification of palmatine as an inhibitor of West Nile virus.

机构信息

State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China.

出版信息

Arch Virol. 2010 Aug;155(8):1325-9. doi: 10.1007/s00705-010-0702-4. Epub 2010 May 23.

PMID:20496087

Abstract

In this study, we investigated the specific inhibition of West Nile virus (WNV) NS2B-NS3 protease and viral propagation by palmatine, a chemical compound from Coptis chinensis Franch. It was demonstrated that palmatine could inhibit WNV NS2B-NS3 protease activity in an uncompetitive manner, with a 50% inhibitory concentration (IC(50)) of 96 microM. Palmatine suppressed WNV without detectable cytotoxicity (a 50% effective concentration [EC(50)] of 3.6 microM and a 50% cytotoxicity concentration [CC(50)] of 1,031 microM). Furthermore, palmatine could also suppress dengue virus and yellow fever virus in a dose-dependent manner. This compound could potentially be developed for the treatment of flavivirus infections.

摘要

在这项研究中，我们研究了小檗碱（一种来自黄连的化合物）对西尼罗河病毒（WNV）NS2B-NS3 蛋白酶和病毒繁殖的特异性抑制作用。研究表明，小檗碱可以以非竞争性方式抑制 WNV NS2B-NS3 蛋白酶活性，其 50%抑制浓度（IC（50））为 96 μM。小檗碱抑制 WNV 而没有检测到细胞毒性（50%有效浓度[EC（50）]为 3.6 μM，50%细胞毒性浓度[CC（50）]为 1031 μM）。此外，小檗碱还可以剂量依赖性方式抑制登革热病毒和黄热病病毒。这种化合物有可能被开发用于治疗黄病毒感染。