Yang Jian-Song, Meng Qing-Xiang, Ren Li-Ping, Zhou Zhen-Ming, Xie Xiang-Xue
State Key Laboratory of Animal Nutrition, College of Animal Science & Technology, China Agricultural University, Beijing 100094, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2010 Mar;30(3):685-7.
The aim of the present study was to develop a near-infrared reflectance (NIR) spectroscopy rapid method for evaluation of beef quality. Partial least squares (PLS) prediction model for the physic-chemical characteristics such as moisture, fat, protein, pH, color and WBSF in beef was established with good veracity. One hundred fourteen samples from five different parts of beef carcass (tenderloin, ribeye, topside, shin, striploin) were collected from meat packer after 48 h aging. Spectra were obtained by scanning sample from 950 to 1 650 nm and pretreated the model by MSC, SNV and first derivative. Predictive correlation coefficients of physic-chemical parameters in beef were 0.947 2 (moisture), 0.924 5 (fat), 0.934 6 (protein), 0.620 2 (pH), 0.820 3 (L), 0.864 6 (a*), 0.753 0 (b*) and 0.475 9 (WBSF) respectively. Root mean square errors of calibration (RMSEC) were 0.313 3 (moisture), 0.221 0 (fat), 1.243 2 (protein), 0.744 6 (pH), 1.778 3 (L*), 1.394 2 (a*), 1.763 9 (b*) and 1.0743 (WBSF). They were externally validated with additional 30 beef samples. Statistics showed that there was no significant difference between predicted value and those obtained with conventional laboratory methods. The results showed that NIRS is a rapid, effective technique for evaluating beef quality. The predictions for chemical characteristics gave higher accuracy than prediction for physical characteristics.
本研究的目的是开发一种用于评估牛肉品质的近红外反射光谱(NIR)快速方法。建立了牛肉中水分、脂肪、蛋白质、pH值、颜色和剪切力等理化特性的偏最小二乘法(PLS)预测模型,准确性良好。在肉品包装商处采集了48小时成熟后的114个来自牛胴体五个不同部位(里脊、肋眼、臀肉、小腿、西冷)的样本。通过在950至1650nm范围内扫描样本获得光谱,并通过多元散射校正(MSC)、标准正态变量变换(SNV)和一阶导数对模型进行预处理。牛肉中理化参数的预测相关系数分别为0.9472(水分)、0.9245(脂肪)、0.9346(蛋白质)、0.6202(pH值)、0.8203(亮度值L)、0.8646(红度值a*)、0.7530(黄度值b*)和0.4759(剪切力WBSF)。校正均方根误差(RMSEC)分别为0.3133(水分)、0.2210(脂肪)、1.2432(蛋白质)、0.7446(pH值)、1.7783(L*)、1.3942(a*)、1.7639(b*)和1.0743(WBSF)。用另外30个牛肉样本进行外部验证。统计结果表明,预测值与传统实验室方法获得的值之间没有显著差异。结果表明,近红外光谱法是一种评估牛肉品质的快速、有效技术。化学特性的预测比物理特性的预测具有更高的准确性。