Appleyard M E, Smith A D
University Department of Pharmacology, South Parks Road, Oxford, OX1 3QT, U.K.
Neurochem Int. 1987;11(4):397-406. doi: 10.1016/0197-0186(87)90029-5.
Spontaneous and evoked secretion of acetylcholinesterase from the hippocampus in vivo has been demonstrated by the use of push-pull cannulae. Local perfusion with 10(?5)M carbachol evoked an increase of 104% in acetylcholinesterase release with no accompanying change in butyrylcholinesterase or lactic dehydrogenase. Local or systemic atropine sulphate blocked the carbachol-evoked increase in acetylcholinesterase release, whilst gallamine had no effect. Local perfusion of ?-aminobutyric acid (10(?4)M) also blocked the carbachol-evoked release of acetylcholinesterase but had no effect on the spontaneous release. It is concluded that a soluble form of acetylcholinesterase is secreted from the hippocampus in response to stimulation of muscarinic receptors; this secretion can be influenced by ?-aminobutyric acid, which is present in interneurones in the hippocampus.
使用推挽式套管已证实在体状态下，海马体能自发分泌及诱发分泌乙酰胆碱酯酶。用10（-5）M氨甲酰胆碱进行局部灌注可使乙酰胆碱酯酶释放增加104%，而丁酰胆碱酯酶或乳酸脱氢酶却无相应变化。局部或全身使用硫酸阿托品可阻断氨甲酰胆碱诱发的乙酰胆碱酯酶释放增加，而加拉明则无此作用。局部灌注γ-氨基丁酸（10（-4）M）也可阻断氨甲酰胆碱诱发的乙酰胆碱酯酶释放，但对自发释放无影响。得出的结论是，可溶性形式的乙酰胆碱酯酶会因毒蕈碱受体受到刺激而从海马体中分泌出来；这种分泌会受到γ-氨基丁酸的影响，γ-氨基丁酸存在于海马体的中间神经元中。
