[Hath1基因转移在体外抑制结肠癌细胞的增殖]

[Hath1 gene transfer inhibits the proliferation of colonic cancer cells in vitro].

作者信息

Zhu Dai-hua, Gong Jian-ping, Ren Ke, Sun Jian-ming, Wei Si-dong

机构信息

Department of General Surgery, Second Hospital of Chongqing University of Medical Sciences, Chongqing 400010, China.

出版信息

Nan Fang Yi Ke Da Xue Xue Bao. 2010 May;30(5):1005-8.

PMID:20501407

Abstract

OBJECTIVE

To study the effect of Hath1 gene transfer on the proliferation of colonic cancer cells in vitro.

METHODS

The recombinant plasmid pcDNA3.1(+)-Hath1 was transfected into HT29 colonic cancer cells, and 3 positive cell clones were randomly selected to test the levels of Hath1 mRNA, Muc2 mRNA, Hath1, Muc2, cyclin D1 and p27 by quantitative real-time RT-PCR and Western blotting. The proliferation of the transfected HT29 cells was observed by means of colony formation assay and xenograft growth in nude mice.

RESULTS

Hath1 significantly down-regulated of cyclin D1 and up-regulate of p27 expressions and inhibited the proliferation of HT29 cells.

CONCLUSION

Hath1 gene may be an anti-oncogene in colon carcinogenesis.

摘要

目的

研究Hath1基因转染对结肠癌细胞体外增殖的影响。

方法

将重组质粒pcDNA3.1(+)-Hath1转染至HT29结肠癌细胞，随机选取3个阳性细胞克隆，采用定量实时逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法检测Hath1 mRNA、Muc2 mRNA、Hath1、Muc2、细胞周期蛋白D1（cyclin D1）和p27的表达水平。通过集落形成实验和裸鼠体内移植瘤生长观察转染后HT29细胞的增殖情况。