异丙酚对心肌β-肾上腺素能受体介导的信号转导的影响；cAMP 的作用。
Effects of propofol on beta-adrenoceptor-mediated signal transduction in cardiac muscle; role of cAMP.
机构信息
Department of Anesthesiology and Pain Medicine, and Anesthesia and Pain Research Institute, Yonsei University College of Medicine, Seoul, Korea.
出版信息
Korean J Anesthesiol. 2010 Apr;58(4):374-7. doi: 10.4097/kjae.2010.58.4.374. Epub 2010 Apr 28.
BACKGROUND
Propofol may decrease myocardial contractility via actions on the beta-adrenoceptor-mediated signal transduction. The aim of this study was to evaluate the effect of propofol via beta-adrenoceptor-mediated signal transduction by measuring the tissue levels of cAMP (cyclic adenosine monophosphate).
METHODS
The effects of propofol on beta-adrenoceptor mediated cascades were measured with cAMP concentrations, which were stimulated by agonists (l-isoproterenol, GTPgammaS, and forskolin) of each step of beta-adrenoceptor-mediated cascades.
RESULTS
While the production of cAMP stimulated by isoproterenol, GTPgammaS, or forskolin are increased (P < 0.05), application of each concentration of propofol (0.1, 1, 10, 100 microM) did not alter the levels of cAMP.
CONCLUSIONS
Considering that propofol did not alter the tissue cAMP levels when stimulated by isoproterenol, GTPgammaS, and forskolin, propofol appears to have no effect on the beta-adrenoceptor signaling pathway in guinea pig ventricular myocardium.
背景
丙泊酚可能通过作用于β-肾上腺素能受体介导的信号转导来降低心肌收缩力。本研究旨在通过测量环磷酸腺苷（cAMP）的组织水平来评估丙泊酚通过β-肾上腺素能受体介导的信号转导的作用。
方法
通过 cAMP 浓度测量丙泊酚对β-肾上腺素能受体介导的级联反应的影响，该浓度由β-肾上腺素能受体介导的级联反应的每个步骤的激动剂（l-异丙肾上腺素、GTPγS 和 forskolin）刺激。
结果
虽然异丙肾上腺素、GTPγS 或福司柯林刺激的 cAMP 产生增加（P<0.05），但应用每种浓度的丙泊酚（0.1、1、10、100μM）均未改变 cAMP 水平。
结论
考虑到异丙肾上腺素、GTPγS 和福司柯林刺激时丙泊酚未改变组织 cAMP 水平，丙泊酚似乎对豚鼠心室心肌的β-肾上腺素能信号通路没有影响。
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