一种简单的荧光策略，用于通过量子点-凝集素纳米探针原位评估细胞表面碳水化合物。
A simple fluorescent strategy for in situ evaluation of cell surface carbohydrate with a quantum dot-lectin nanoprobe.
机构信息
Key Laboratory of Analytical Chemistry for Life Science (Ministry of Education of China), Department of Chemistry, Nanjing University, Nanjing 210093, P. R. China.
出版信息
Analyst. 2010 Aug;135(8):1906-8. doi: 10.1039/c0an00250j. Epub 2010 Jun 1.
PMID:20514385
Abstract
A simple and rapid fluorescent method was developed for the in situ evaluation of cell surface carbohydrate by homogeneous specific recognition of a quantum dot-lectin nanoprobe to mannosyl groups on the cell surface. The strategy was further used for dynamically monitoring the alteration of cell surface carbohydrate expression in response to drugs.
摘要
建立了一种简单快速的荧光方法，通过量子点-凝集素纳米探针对细胞表面甘露糖基的同型特异性识别，实现了对细胞表面碳水化合物的原位评价。该策略进一步用于动态监测细胞表面碳水化合物表达在药物作用下的变化。
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