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实时逆转录聚合酶链反应与病毒分离法在估算德克萨斯州墨西哥湾中部海岸水鸟越冬地猎人捕获的野生鸟类中禽流感病毒流行率方面的比较(2005 - 2006年至2008 - 2009年)

Comparison of real-time reverse transcription-PCR and virus isolation for estimating prevalence of avian influenza virus in hunter-harvested wild birds at waterfowl wintering grounds along the Texas mid-Gulf Coast (2005-2006 through 2008-2009).

作者信息

Ferro Pamela J, Peterson Markus J, Merendino Todd, Nelson Matt, Lupiani Blanca

机构信息

Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX 77843, USA.

出版信息

Avian Dis. 2010 Mar;54(1 Suppl):655-9. doi: 10.1637/8810-040109-ResNote.1.

Abstract

Historically, virus isolation has been the method of choice for conducting surveillance for avian influenza virus (AIV) in avian species. More recently, the primary screening method has become real-time reverse transcription-polymerase chain reaction (RRT-PCR). We wanted to determine how these two testing methods (virus isolation and RRT-PCR) affected AIV prevalence estimation, particularly in an understudied, low-prevalence region-the waterfowl wintering grounds along the Texas mid-Gulf Coast. Cloacal swabs were collected from hunter-harvested waterfowl and other wetland-associated game birds during four consecutive hunting seasons (2005-2006 through 2008-2009). Overall prevalence by RRT-PCR (5.9%, 6.5%, 11.2%, and 5.5%) was approximately an order of magnitude higher than prevalence by virus isolation (0.5%, 1.3%, 3.9%, and 0.7%) for the four hunting seasons, respectively. Apparent AIV prevalence by virus isolation conducted only on RRT-PCR-positive samples resulted in estimates nearly identical in magnitude to those derived from parallel testing (0.5% vs. 0.6%, 1.3% vs. 1.7%, and 3.9% vs. 4.0% for 2005-2006, 2006-2007, and 2007-2008, respectively). Unlike most reports of seasonal variation in AIV prevalence, we documented differences in prevalence estimates among months by RRT-PCR only during 2008-2009 and by virus isolation only during 2006-2007 and 2007-2008. Our data indicate that screening samples by RRT-PCR followed by virus isolation only on RRT-PCR-positive samples provides a reasonable means to generate prevalence estimates close to the true prevalence as determined by virus isolation. We also confirmed the low prevalence of AIV in waterfowl wintering grounds along the Texas mid-Gulf Coast and demonstrated little variation in prevalence among months during the four hunting seasons sampled.

摘要

从历史上看,病毒分离一直是对禽类中的禽流感病毒(AIV)进行监测的首选方法。最近,主要的筛查方法已变为实时逆转录聚合酶链反应(RRT-PCR)。我们想确定这两种检测方法(病毒分离和RRT-PCR)如何影响AIV流行率估计,特别是在一个研究较少、低流行率的地区——得克萨斯州墨西哥湾中部海岸的水鸟越冬地。在连续四个狩猎季节(2005 - 2006年至2008 - 2009年)期间,从猎人捕获的水鸟和其他与湿地相关的猎鸟中采集泄殖腔拭子。在这四个狩猎季节中,RRT-PCR检测的总体流行率(分别为5.9%、6.5%、11.2%和5.5%)比病毒分离检测的流行率(分别为0.5%、

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