嗜酸性粒细胞是 BALB/c 背景下 Th 转移模型中诱导支气管高反应性所必需的。
Eosinophils are required for the induction of bronchial hyperresponsiveness in a Th transfer model of BALB/c background.
机构信息
National Hospital Organization, Sagamihara National Hospital, Clinical Research Center for Allergy and Rheumatology, Kanagawa, Japan.
出版信息
Int Arch Allergy Immunol. 2010;152 Suppl 1:79-82. doi: 10.1159/000312130. Epub 2010 Jun 4.
BACKGROUND
Helper T (Th) cells are deeply involved in the pathophysiology of bronchial asthma, such as eosinophilic inflammation, bronchial hyperresponsiveness (BHR), airflow limitation and remodeling. It is still unclear whether Th cells contribute to BHR independently of eosinophilic inflammation. The double GATA (dblGATA) site is a high-affinity GATA-binding site in the GATA-1 promoter. dblGATA site-deficient (Delta dblGATA) mice lack eosinophils.
METHOD
Ovalbumin (OVA)-reactive Th clones were transferred into Delta dblGATA and wild-type (WT) mice of BALB/c background. The number of eosinophils in the bronchoalveolar lavage fluid (BALF) and bronchial responsiveness to methacholine were examined after OVA challenge.
RESULTS
The number of BALF eosinophils was significantly increased in WT mice, but not detectable in Delta dblGATA mice. BHR was also induced in WT mice, but significantly attenuated in Delta dblGATA mice.
CONCLUSION
Eosinophils are involved in T-cell-mediated BHR.
背景
辅助性 T（Th）细胞深入参与支气管哮喘的病理生理学，如嗜酸性粒细胞炎症、支气管高反应性（BHR）、气流受限和重塑。目前尚不清楚 Th 细胞是否独立于嗜酸性粒细胞炎症而导致 BHR。双 GATA（dblGATA）位点是 GATA-1 启动子中的高亲和力 GATA 结合位点。dblGATA 位点缺失（Delta dblGATA）小鼠缺乏嗜酸性粒细胞。
方法
卵清蛋白（OVA）反应性 Th 克隆被转移到 Delta dblGATA 和 BALB/c 背景的野生型（WT）小鼠中。OVA 挑战后，检测支气管肺泡灌洗液（BALF）中的嗜酸性粒细胞数量和对乙酰甲胆碱的支气管反应性。
结果
WT 小鼠的 BALF 嗜酸性粒细胞数量显著增加，但在 Delta dblGATA 小鼠中则无法检测到。WT 小鼠也诱导了 BHR，但在 Delta dblGATA 小鼠中则显著减弱。
结论
嗜酸性粒细胞参与 T 细胞介导的 BHR。
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