Ding Weiping, Zhou Xiaoming, Heimfeld Shelly, Reems Jo-Anna, Gao Dayong
Department of Mechanical Engineering, University of Washington, Seattle, WA 98195, USA.
J Biomech Eng. 2010 Jan;132(1):011002. doi: 10.1115/1.4000110.
Hollow fiber modules are commonly used to conveniently and efficiently remove cryoprotective agents (CPAs) from cryopreserved cell suspensions. In this paper, a steady-state model coupling mass transfers across cell and hollow fiber membranes is theoretically developed to evaluate the removal of CPAs from cryopreserved blood using hollow fiber modules. This steady-state model complements the unsteady-state model, which was presented in our previous study. The steady-state model, unlike the unsteady-state model, can be used to evaluate the effect of ultrafiltration flow rates on the clearance of CPAs. The steady-state model is validated by experimental results, and then is compared with the unsteady-state model. Using the steady-state model, the effects of ultrafiltration flow rates, NaCl concentrations in dialysate, blood flow rates and dialysate flow rates on CPA concentration variation and cell volume response are investigated in detail. According to the simulative results, the osmotic damage of red blood cells can easily be reduced by increasing ultrafiltration flow rates, increasing NaCl concentrations in dialysate, increasing blood flow rates, or decreasing dialysate flow rates.
中空纤维模块通常用于方便、高效地从冷冻保存的细胞悬液中去除冷冻保护剂（CPA）。本文从理论上建立了一个耦合细胞与中空纤维膜传质的稳态模型，以评估使用中空纤维模块从冷冻保存的血液中去除CPA的效果。该稳态模型补充了我们之前研究中提出的非稳态模型。与非稳态模型不同，稳态模型可用于评估超滤流速对CPA清除率的影响。通过实验结果对稳态模型进行了验证，并与非稳态模型进行了比较。利用稳态模型，详细研究了超滤流速、透析液中NaCl浓度、血流速度和透析液流速对CPA浓度变化和细胞体积响应的影响。根据模拟结果，通过提高超滤流速、提高透析液中NaCl浓度、提高血流速度或降低透析液流速，可以很容易地降低红细胞的渗透损伤。
