环介导等温扩增法检测肠聚集性大肠杆菌。
Detection of enteroaggregative Escherichia coli by loop-mediated isothermal amplification.
机构信息
Division of Bacteriology, Chiba Prefectural Institute of Public Health, 666-2, Chuo, Chiba City, Chiba 260-8715, Japan.
出版信息
J Food Prot. 2010 Jun;73(6):1064-72. doi: 10.4315/0362-028x-73.6.1064.
A novel gene amplification method, loop-mediated isothermal amplification (LAMP), has been recently developed as a rapid, specific diagnostic method for various infectious diseases. We have investigated whether LAMP can be used to detect small numbers of enteroaggregative Escherichia coli (EAEC) cells contaminated in food samples. Primers for LAMP reaction were designed with EAEC aggR gene sequences (available in GenBank). LAMP specificity with these primers was the same as that of PCR in a study of 37 EAEC and 42 non-EAEC bacterial strains. The sensitivity of the LAMP method was better than that of PCR in a study of serially diluted EAEC cells. The LAMP method was significantly more effective than was PCR in detecting EAEC-contaminated food samples (Fisher's exact test, P < 0.05). Therefore, the LAMP method described here should be useful for detecting small numbers of EAEC cells in food samples.
一种新的基因扩增方法，环介导等温扩增（LAMP），最近已被开发为一种快速、特异的诊断各种传染病的方法。我们研究了 LAMP 是否可用于检测食品样本中污染的少量肠集聚性大肠杆菌（EAEC）细胞。LAMP 反应的引物是根据 EAEC aggR 基因序列设计的（可在 GenBank 中获得）。在对 37 株 EAEC 和 42 株非 EAEC 细菌株的研究中，这些引物的 LAMP 特异性与 PCR 相同。在对连续稀释的 EAEC 细胞的研究中，LAMP 方法的灵敏度优于 PCR。LAMP 方法在检测 EAEC 污染的食品样本方面明显优于 PCR（Fisher 确切检验，P < 0.05）。因此，这里描述的 LAMP 方法应该有助于检测食品样本中的少量 EAEC 细胞。
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